PDF DatasheetHuman Ubiquitin-conjugating enzyme E2 S Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant Ubiquitin-conjugating enzyme E2 S based upon sequence from: Human
Host: QP7603 protein expressed in E. coli.
Tag: GST
Protein Construction: A DNA sequence encoding the Homo sapiens (Human) Ubiquitin-conjugating enzyme E2 S, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Application Notes: Please contact us for application specific information for QP7603.
Bioactivity Data: Untested
Full Length? Full Length
Expression Region: Met1 – Leu222
Amino Acid Sequence: MNSNVENLPP HIIRLVYKEV TTLTADPPDG IKVFPNEEDL TDLQVTIEGP EGTPYAGGLF RMKLLLGKDF PASPPKGYFL TKIFHPNVGA NGEICVNVLK RDWTAELGIR HVLLTIKCLL IHPNPESALN EEAGRLLLEN YEEYAARARL LTEIHGGAGG PSGRAEAGRA LASGTEASST DPGAPGGPGG AEGPMAKKHA GERDKKLAAK KKTDKKRALR RL
Purity: Greater than 80% as determined by SDS-PAGE.
Reconstitution Instructions:
Concentration of Human Ubiquitin-conjugating enzyme E2 S Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant Human Ubiquitin-conjugating enzyme E2 S Protein General Information | |
|---|---|
| Alternate Names | |
| EPF5; E2EPF; E2-EPF | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | UBE2S |
| Entrez Gene ID | 27338 |
| Ensemble Gene ID | ENSG00000108106 |
| RefSeq Protein Accession(s) | NP_055316.2 |
| RefSeq mRNA Accession(s) | NM_014501.2 |
| UniProt ID(s) | Q16763 |
| UniGene ID(s) | Hs.396393 |
| HGNC ID(s) | HGNC:17895 |
| COSMIC ID Link(s) | UBE2S |
| KEGG Gene ID(s) | hsa:27338 |
| PharmGKB ID(s) | PA134904441 |
| General Description of Recombinant Human Ubiquitin-conjugating enzyme E2 S Protein. | |
| Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes ‘Lys-11’-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by specifically elongating ‘Lys-11’-linked polyubiquitin chains initiated by the E2 enzyme UBE2C/UBCH10 on APC/C substrates, enhancing the degradation of APC/C substrates by the proteasome and promoting mitotic exit. Also acts by elongating ubiquitin chains initiated by the E2 enzyme UBE2D1/UBCH5 in vitro; it is however unclear whether UBE2D1/UBCH5 acts as an E2 enzyme for the APC/C in vivo. Also involved in ubiquitination and subsequent degradation of VHL, resulting in an accumulation of HIF1A. In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, except ‘Lys-48’-linked polyubiquitination. | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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