PDF DatasheetHuman Sorting nexin-1 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant Sorting nexin-1 based upon sequence from: Human
Host: QP7609 protein expressed in E. coli.
Tag: His-SUMO
Protein Construction: A DNA sequence encoding the Homo sapiens (Human) Sorting nexin-1, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Application Notes: Please contact us for application specific information for QP7609.
Bioactivity Data: Untested
Full Length? Full Length
Expression Region: Met1 – Ser522
Amino Acid Sequence: MASGGGGCSA SERLPPPFPG LEPESEGAAG GSEPEAGDSD TEGEDIFTGA AVVSKHQSPK ITTSLLPINN GSKENGIHEE QDQEPQDLFA DATVELSLDS TQNNQKKVLA KTLISLPPQE ATNSSKPQPT YEELEEEEQE DQFDLTVGIT DPEKIGDGMN AYVAYKVTTQ TSLPLFRSKQ FAVKRRFSDF LGLYEKLSEK HSQNGFIVPP PPEKSLIGMT KVKVGKEDSS SAEFLEKRRA ALERYLQRIV NHPTMLQDPD VREFLEKEEL PRAVGTQTLS GAGLLKMFNK ATDAVSKMTI KMNESDIWFE EKLQEVECEE QRLRKLHAVV ETLVNHRKEL ALNTAQFAKS LAMLGSSEDN TALSRALSQL AEVEEKIEQL HQEQANNDFF LLAELLSDYI RLLAIVRAAF DQRMKTWQRW QDAQATLQKK REAEARLLWA NKPDKLQQAK DEILEWESRV TQYERDFERI STVVRKEVIR FEKEKSKDFK NHVIKYLETL LYSQQQLAKY WEAFLPEAKA IS
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions:
Concentration of Human Sorting nexin-1 Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant Human Sorting nexin-1 Protein General Information | |
|---|---|
| Alternate Names | |
| VPS5; HsT17379 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | SNX1 |
| Entrez Gene ID | 6642 |
| Ensemble Gene ID | ENSG00000028528 |
| RefSeq Protein Accession(s) | NP_001229862.1 |
| RefSeq mRNA Accession(s) | NM_001242933.1, NM_003099.4, NM_148955.3 |
| UniProt ID(s) | Q13596 |
| UniGene ID(s) | Hs.188634 |
| HGNC ID(s) | HGNC:11172 |
| COSMIC ID Link(s) | SNX1 |
| KEGG Gene ID(s) | hsa:6642 |
| PharmGKB ID(s) | PA36011 |
| General Description of Recombinant Human Sorting nexin-1 Protein. | |
| Involved in several stages of intracellular trafficking. Interacts with mbranes containing phosphatidylinositol 3-phosphate (PtdIns(3P)) or phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2) . Acts in part as component of the retromer mbrane-deforming SNX-BAR subcomplex. The SNX-BAR retromer mediates retrograde transport of cargo proteins from endosomes to the trans-Golgi network (TGN) and is involved in endosome-to-plasma mbrane transport for cargo protein recycling. The SNX-BAR subcomplex functions to deform the donor mbrane into a tubular profile called endosome-to-TGN transport carrier (ETC) (Probable). Can sense mbrane curvature and has in vitro vesicle-to-mbrane rodeling activity . Involved in retrograde endosome-to-TGN transport of lysosomal enzyme receptors (IGF2R, M6PR and SORT1) and Shiginella dysenteria toxin stxB. Plays a role in targeting ligand-activated EGFR to the lysosomes for degradation after endocytosis from the cell surface and release from the Golgi . Involvent in retromer-independent endocytic trafficking of P2RY1 and lysosomal degradation of protease-activated receptor-1/F2R . Promotes KALRN- and RHOG-dependent but retromer-independent mbrane rodeling such as lamellipodium formation; the function is dependent on GEF activity of KALRN . Required for endocytosis of DRD5 upon agonist stimulation but not for basal receptor trafficking . | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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