PDF DatasheetDog Epididymal secretory protein E1 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant Epididymal secretory protein E1 based upon sequence from: Dog
Host: QP7631 protein expressed in E.coli, Yeast..
Tag: His
Protein Construction: A DNA sequence encoding the Canis lupus familiaris (Dog) (Canis familiaris) Epididymal secretory protein E1, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Application Notes: Please contact us for application specific information for QP7631.
Bioactivity Data: Untested
Full Length? Full Length
Expression Region: Val22 – Gly149
Amino Acid Sequence: VHFKDCGSAV GVIKELNVNP CPAQPCKLHK GQSYSVNVTF TSNIPSQSSK AVVHGIVLGV AVPFPIPEAD GCKSGINCPI QKDKTYSYLN KLPVKNEYPS IKLVVQWMLL GDNNQHLFCW EIPVQIEG
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions:
Concentration of Dog Epididymal secretory protein E1 Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant Canine Epididymal secretory protein E1 Protein General Information | |
|---|---|
| Alternate Names | |
| CE1 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | NPC |
| UniProt ID(s) | Q28895 |
| UniGene ID(s) | Cfa.3781 |
| General Description of Recombinant Canine Epididymal secretory protein E1 Protein. | |
| Intracellular cholesterol transporter which acts in concert with NPC1 and plays an important role in the egress of cholesterol from the endosomal/lysosomal compartment. Both NPC1 and NPC2 function as the cellular ‘tag team duo’ (TTD) to catalyze the mobilization of cholesterol within the multivesicular environment of the late endosome (LE) to effect egress through the limiting bilayer of the LE. NPC2 binds unesterified cholesterol that has been released from LDLs in the lumen of the late endosomes/lysosomes and transfers it to the cholesterol-binding pocket of the N-terminal domain of NPC1. Cholesterol binds to NPC1 with the hydroxyl group buried in the binding pocket and is exported from the limiting mbrane of late endosomes/ lysosomes to the ER and plasma mbrane by an unknown mechanism. The secreted form of NCP2 regulates biliary cholesterol secretion via stimulation of ABCG5/ABCG8-mediated cholesterol transport . | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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