PDF DatasheetRat Cytochrome P450 1B1 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant Cytochrome P450 1B1 based upon sequence from: Rat
Host: QP7721 protein expressed in E. coli.
Tag: His
Protein Construction: A DNA sequence encoding the Rattus norvegicus (Rat) Cytochrome P450 1B1, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Application Notes: Please contact us for application specific information for QP7721.
Bioactivity Data: Untested
Full Length? Full Length
Expression Region: Met1 – Gln543
Amino Acid Sequence: MATSLSADSP QQLSSLSTQQ TILLLLVSVL AIVHLGQWLL RQWRRKPWSS PPGPFPWPLI GNAASVGRAS HLYFARLARR YGDVFQIRLG SCPVVVLNGE SAIHQALVQQ GGVFADRPPF ASFRVVSGGR SLAFGHYSER WKERRRAAYG TMRAFSTRHP RSRGLLEGHA LGEARELVAV LVRRCAGGAC LDPTQPIIVA VANVMSAVCF GCRYNHDDAE FLELLSHNEE FGRTVGAGSL VDVMPWLQLF PNPVRTIFRE FEQINRNFSN FVLDKFLRHR ESLVPGAAPR DMMDAFILSA EKKATGDPGD SPSGLDLEDV PATITDIFGA SQDTLSTALL WLLILFTRYP DVQARVQAEL DQVVGRDRLP CMSDQPNLPY VMAFLYESMR FTSFLPVTLP HATTANTFVL GYYIPKNTVV FVNQWSVNHD PAKWSNPEDF DPARFLDKDG FINKALASSV MIFSVGKRRC IGEELSKTLL FLFISILAHQ CNFKANQNEP SNMSFSYGLS IKPKSFKIHV SLRESMKLLD SAVEKLQAEE ACQ
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions:
Concentration of Rat Cytochrome P450 1B1 Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant Rat Cytochrome P450 1B1 Protein General Information | |
|---|---|
| Curated Database and Bioinformatic Data | |
| Gene Symbol | Cyp1b1 |
| Entrez Gene ID | 25426 |
| RefSeq Protein Accession(s) | NP_037072.1 |
| RefSeq mRNA Accession(s) | NM_012940.2 |
| UniProt ID(s) | Q64678 |
| UniGene ID(s) | Rn.10125 |
| COSMIC ID Link(s) | Cyp1b1 |
| KEGG Gene ID(s) | rno:25426 |
| General Description of Recombinant Rat Cytochrome P450 1B1 Protein. | |
| Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, retinoid and xenobiotics. Preferentially oxidizes 17beta-estradiol to the carcinogenic 4-hydroxy derivative, and a variety of procarcinogenic compounds to their activated forms, including polycyclic aromatic hydrocarbons. Promotes angiogenesis by removing cellular oxygenation products, thereby decreasing oxidative stress, release of antiangiogenic factor THBS2, then allowing endothelial cells migration, cell adhesion and capillary morphogenesis. These changes are concommitant with the endothelial nitric oxide synthase activity and nitric oxide synthesis. Plays an important role in the regulation of perivascular cell proliferation, migration, and survival through modulation of the intracellular oxidative state and NF-kappa-B expression and/or activity, during angiogenesis. Contributes to oxidative homeostasis and ultrastructural organization and function of trabecular meshwork tissue through modulation of POSTN expression. | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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