PDF DatasheetMouse NPM1 / Nucleophosmin Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant NPM1 / Nucleophosmin based upon sequence from: Mouse
Host: QP7731 protein expressed in E. coli.
Tag: His-SUMO
Protein Construction: A DNA sequence encoding the Mus musculus (Mouse) NPM1 / Nucleophosmin, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Application Notes: Please contact us for application specific information for QP7731.
Bioactivity Data: Untested
Full Length? Full Length
Expression Region: Met1 – Leu292
Amino Acid Sequence: MEDSMDMDMS PLRPQNYLFG CELKADKDYH FKVDNDENEH QLSLRTVSLG AGAKDELHIV EAEAMNYEGS PIKVTLATLK MSVQPTVSLG GFEITPPVVL RLKCGSGPVH ISGQHLVAVE EDAESEDEDE EDVKLLGMSG KRSAPGGGNK VPQKKVKLDE DDEDDDEDDE DDEDDDDDDF DEEETEEKVP VKKSVRDTPA KNAQKSNQNG KDLKPSTPRS KGQESFKKQE KTPKTPKGPS SVEDIKAKMQ ASIEKGGSLP KVEAKFINYV KNCFRMTDQE AIQDLWQWRK SL
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions:
Concentration of Mouse NPM1 / Nucleophosmin Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant Mouse NPM1 / Nucleophosmin Protein General Information | |
|---|---|
| Curated Database and Bioinformatic Data | |
| Gene Symbol | Npm1 |
| Entrez Gene ID | 18148 |
| Ensemble Gene ID | ENSMUSG00000057113 |
| RefSeq Protein Accession(s) | NP_032748.1 |
| RefSeq mRNA Accession(s) | NM_008722.3 |
| UniProt ID(s) | Q61937 |
| UniGene ID(s) | Mm.485384 |
| COSMIC ID Link(s) | Npm1 |
| KEGG Gene ID(s) | mmu:18148 |
| General Description of Recombinant Mouse NPM1 / Nucleophosmin Protein. | |
| Involved in diverse cellular processes such as ribosome biogenesis, centrosome duplication, protein chaperoning, histone assembly, cell proliferation, and regulation of tumor suppressors p53/TP53 and ARF. Binds ribosome presumably to drive ribosome nuclear export. Associated with nucleolar ribonucleoprotein structures and bind single-stranded nucleic acids. Acts as a chaperonin for the core histones H3, H2B and H4. Stimulates APEX1 endonuclease activity on apurinic/apyrimidinic (AP) double-stranded DNA but inhibits APEX1 endonuclease activity on AP single-stranded RNA. May exert a control of APEX1 endonuclease activity within nucleoli devoted to repair AP on rDNA and the removal of oxidized rRNA molecules. In concert with BRCA2, regulates centrosome duplication. Regulates centriole duplication: phosphorylation by PLK2 is able to trigger centriole replication. Negatively regulates the activation of EIF2AK2/PKR and suppresses apoptosis through inhibition of EIF2AK2/PKR autophosphorylation. Antagonizes the inhibitory effect of ATF5 on cell proliferation and relieves ATF5-induced G2/M blockade. | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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