PDF DatasheetHuman ATG14 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant ATG14 based upon sequence from: Human
Host: QP7740 protein expressed in E. coli.
Tag: His-Myc
Protein Construction: A DNA sequence encoding the Homo sapiens (Human) ATG14, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Application Notes: Please contact us for application specific information for QP7740.
Bioactivity Data: Untested
Full Length? Full Length
Expression Region: Met1 – Arg492
Amino Acid Sequence: MASPSGKGAR ALEAPGCGPR PLARDLVDSV DDAEGLYVAV ERCPLCNTTR RRLTCAKCVQ SGDFVYFDGR DRERFIDKKE RLSRLKSKQE EFQKEVLKAM EGKWITDQLR WKIMSCKMRI EQLKQTICKG NEEMEKNSEG LLKTKEKNQK LYSRAQRHQE KKEKIQRHNR KLGDLVEKKT IDLRSHYERL ANLRRSHILE LTSVIFPIEE VKTGVRDPAD VSSESDSAMT SSTVSKLAEA RRTTYLSGRW VCDDHNGDTS ISITGPWISL PNNGDYSAYY SWVEEKKTTQ GPDMEQSNPA YTISAALCYA TQLVNILSHI LDVNLPKKLC NSEFCGENLS KQKFTRAVKK LNANILYLCF SQHVNLDQLQ PLHTLRNLMY LVSPSSEHLG RSGPFEVRAD LEESMEFVDP GVAGESDESG DERVSDEETD LGTDWENLPS PRFCDIPSQS VEVSQSQSTQ ASPPIASSSA GGMISSAAAS VTSWFKAYTG HR
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions:
Concentration of Human ATG14 Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant Human ATG14 Protein General Information | |
|---|---|
| Alternate Names | |
| KIAA0831; ATG14L; BARKOR; Beclin 1-associated autophagy-related key regulat | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | ATG14 |
| Entrez Gene ID | 22863 |
| Ensemble Gene ID | ENSG00000126775 |
| RefSeq Protein Accession(s) | NP_055739.2 |
| RefSeq mRNA Accession(s) | NM_014924.4, XM_011536563.2 |
| UniProt ID(s) | Q6ZNE5 |
| UniGene ID(s) | Hs.414809 |
| HGNC ID(s) | HGNC:19962 |
| COSMIC ID Link(s) | ATG14 |
| KEGG Gene ID(s) | hsa:22863 |
| PharmGKB ID(s) | PA165478560 |
| General Description of Recombinant Human ATG14 Protein. | |
| Required for both basal and inducible autophagy. Determines the localization of the autophagy-specific PI3-kinase complex PI3KC3-C1 (PubMed:18843052, PubMed:19050071). Plays a role in autophagosome formation and MAP1LC3/LC3 conjugation to phosphatidylethanolamine (PubMed:19270696, PubMed:20713597). Promotes BECN1 translocation from the trans-Golgi network to autophagosomes (PubMed:20713597). Enhances PIK3C3 activity in a BECN1-dependent manner. Essential for the autophagy-dependent phosphorylation of BECN1 (PubMed:23878393). Stimulates the phosphorylation of BECN1, but suppresses the phosphorylation PIK3C3 by AMPK (PubMed:23878393). Binds to STX17-SNAP29 binary t-SNARE complex on autophagosomes and primes it for VAMP8 interaction to promote autophagosome-endolysosome fusion (PubMed:25686604). Modulates the hepatic lipid metabolism (By similarity). | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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