PDF DatasheetE. coli Mcr-1 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant Mcr-1 based upon sequence from: E. coli
Host: QP7746 protein expressed in E.coli, Invitro-E.coli..
Tag: His-SUMO
Protein Construction: A DNA sequence encoding the Escherichia coli (strain K12) Mcr-1, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Application Notes: Please contact us for application specific information for QP7746.
Bioactivity Data: Untested
Full Length? Full Length
Expression Region: Met1 – Arg541
Amino Acid Sequence: MMQHTSVWYR RSVSPFVLVA SVAVFLTATA NLTFFDKISQ TYPIADNLGF VLTIAVVLFG AMLLITTLLS SYRYVLKPVL ILLLIMGAVT SYFTDTYGTV YDTTMLQNAL QTDQAETKDL LNAAFIMRII GLGVLPSLLV AFVKVDYPTW GKGLMRRLGL IVASLALILL PVVAFSSHYA SFFRVHKPLR SYVNPIMPIY SVGKLASIEY KKASAPKDTI YHAKDAVQAT KPDMRKPRLV VFVVGETARA DHVSFNGYER DTFPQLAKID GVTNFSNVTS CGTSTAYSVP CMFSYLGADE YDVDTAKYQE NVLDTLDRLG VSILWRDNNS DSKGVMDKLP KAQFADYKSA TNNAICNTNP YNECRDVGML VGLDDFVAAN NGKDMLIMLH QMGNHGPAYF KRYDEKFAKF TPVCEGNELA KCEHQSLINA YDNALLATDD FIAQSIQWLQ THSNAYDVSM LYVSDHGESL GENGVYLHGM PNAFAPKEQR SVPAFFWTDK QTGITPMATD TVLTHDAITP TLLKLFDVTA DKVKDRTAFI R
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions:
Concentration of E. coli Mcr-1 Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant E. coli Mcr-1 Protein General Information | |
|---|---|
| Alternate Names | |
| ; Probable phosphatidylethanolamine transferase Mcr | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | mcr1 |
| RefSeq Protein Accession(s) | WP_049589868.1 |
| RefSeq mRNA Accession(s) | NZ_NGVG01000063.1 |
| UniProt ID(s) | A0A0R6L508 |
| General Description of Recombinant E. coli Mcr-1 Protein. | |
| Probably catalyzes the addition of a phosphoethanolamine moiety to lipid A. Phosphoethanolamine modification of lipid A gives polymyxin resistance (PubMed:26603172).1 Publication Confers resistance to polymyxin-type antibiotics; expression of the Mcr-1 protein in E.coli increases colistin and polymyxin B minimal inhibitory concentration (MIC) from 0.5 mg/ml to 2.0 mg/ml. The pHNSHP45 plasmid can transfer efficiently (0.1 to 0.001) to other E.coli strains by conjugation and increases polymxin MIC by 8- to 16-fold; it may not require selective pressure to be maintained in the cell. When transformed into K.pneumoniae or P.aeruginosa it also increases polymxin MIC 8- to 16-fold. In a murine (BALB/c mice) thigh infection study using an mcr1-encoding plasmid isolated from a human patient, the plasmid confers in vivo protection against colistin (PubMed:26603172). |
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Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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