PDF DatasheetHuman E3 ubiquitin-protein ligase SIAH1 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant E3 ubiquitin-protein ligase SIAH1 based upon sequence from: Human
Isoform: Isoform 2
Host: QP7820 protein expressed in E. coli.
Tag: GST
Protein Construction: A DNA sequence encoding the Homo sapiens (Human) E3 ubiquitin-protein ligase SIAH1, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Application Notes: Please contact us for application specific information for QP7820.
Bioactivity Data: Untested
Full Length? Full Length of Isoform 2
Expression Region: Met1 – Cys313
Amino Acid Sequence: MTGKATPPSL YSWRGVLFTC LPAARTRKRK EMSRQTATAL PTGTSKCPPS QRVPALTGTT ASNNDLASLF ECPVCFDYVL PPILQCQSGH LVCSNCRPKL TCCPTCRGPL GSIRNLAMEK VANSVLFPCK YASSGCEITL PHTEKADHEE LCEFRPYSCP CPGASCKWQG SLDAVMPHLM HQHKSITTLQ GEDIVFLATD INLPGAVDWV MMQSCFGFHF MLVLEKQEKY DGHQQFFAIV QLIGTRKQAE NFAYRLELNG HRRRLTWEAT PRSIHEGIAT AIMNSDCLVF DTSIAQLFAE NGNLGINVTI SMC
Purity: Greater than 80% as determined by SDS-PAGE.
Reconstitution Instructions:
Concentration of Human E3 ubiquitin-protein ligase SIAH1 Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant Human E3 ubiquitin-protein ligase SIAH1 Protein General Information | |
|---|---|
| Alternate Names | |
| SIAH1A | |
| Molecular Weight | |
| Isoform 2 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | SIAH1 |
| Entrez Gene ID | 6477 |
| Ensemble Gene ID | ENSG00000196470 |
| RefSeq Protein Accession(s) | NP_001006611.1 |
| RefSeq mRNA Accession(s) | NM_001006610.1, NM_003031.3, XM_006721246.1, XM_011523279.1 |
| UniProt ID(s) | Q8IUQ4 |
| UniGene ID(s) | Hs.706828 |
| HGNC ID(s) | HGNC:10857 |
| COSMIC ID Link(s) | SIAH1 |
| KEGG Gene ID(s) | hsa:6477 |
| PharmGKB ID(s) | PA35759 |
| General Description of Recombinant Human E3 ubiquitin-protein ligase SIAH1 Protein. | |
| E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Mediates E3 ubiquitin ligase activity either through direct binding to substrates or by functioning as the essential RING domain subunit of larger E3 complexes. Triggers the ubiquitin-mediated degradation of many substrates, including proteins involved in transcription regulation (ELL2, MYB, POU2AF1, PML and RBBP8), a cell surface receptor (DCC), the cell-surface receptor-type tyrosine kinase FLT3, the cytoplasmic signal transduction molecules (KLF10/TIEG1 and NUMB), an antiapoptotic protein (BAG1), a microtubule motor protein (KIF22), a protein involved in synaptic vesicle function in neurons (SYP), a structural protein (CTNNB1) and SNCAIP. Confers constitutive instability to HIPK2 through proteasomal degradation. It is thereby involved in many cellular processes such as apoptosis, tumor suppression, cell cycle, axon guidance, transcription regulation, spermatogenesis and TNF-alpha signaling. Has some overlapping function with SIAH2. Induces apoptosis in cooperation with PEG3. Upon nitric oxid (NO) generation that follows apoptotic stimulation, interacts with S-nitrosylated GAPDH, mediating the translocation of GAPDH to the nucleus. GAPDH acts as a stabilizer of SIAH1, facilitating the degradation of nuclear proteins. | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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