PDF DatasheetHuman F-box / WD repeat-containing protein 7 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant F-box / WD repeat-containing protein 7 based upon sequence from: Human
Host: QP7843 protein expressed in E.coli, Invitro-E.coli..
Tag: His
Protein Construction: A DNA sequence encoding the Homo sapiens(Human) F-box/WD repeat-containing protein 7, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Application Notes: Please contact us for application specific information for QP7843.
Bioactivity Data: Untested
Full Length? Full Length
Expression Region: Met1 – Lys707
Amino Acid Sequence: MNQELLSVGS KRRRTGGSLR GNPSSSQVDE EQMNRVVEEE QQQQLRQQEE EHTARNGEVV GVEPRPGGQN DSQQGQLEEN NNRFISVDED SSGNQEEQEE DEEHAGEQDE EDEEEEEMDQ ESDDFDQSDD SSREDEHTHT NSVTNSSSIV DLPVHQLSSP FYTKTTKMKR KLDHGSEVRS FSLGKKPCKV SEYTSTTGLV PCSATPTTFG DLRAANGQGQ QRRRITSVQP PTGLQEWLKM FQSWSGPEKL LALDELIDSC EPTQVKHMMQ VIEPQFQRDF ISLLPKELAL YVLSFLEPKD LLQAAQTCRY WRILAEDNLL WREKCKEEGI DEPLHIKRRK VIKPGFIHSP WKSAYIRQHR IDTNWRRGEL KSPKVLKGHD DHVITCLQFC GNRIVSGSDD NTLKVWSAVT GKCLRTLVGH TGGVWSSQMR DNIIISGSTD RTLKVWNAET GECIHTLYGH TSTVRCMHLH EKRVVSGSRD ATLRVWDIET GQCLHVLMGH VAAVRCVQYD GRRVVSGAYD FMVKVWDPET ETCLHTLQGH TNRVYSLQFD GIHVVSGSLD TSIRVWDVET GNCIHTLTGH QSLTSGMELK DNILVSGNAD STVKIWDIKT GQCLQTLQGP NKHQSAVTCL QFNKNFVITS SDDGTVKLWD LKTGEFIRNL VTLESGGSGG VVWRIRASNT KLVCAVGSRN GTEETKLLVL DFDVDMK
Purity: Greater than 85% as determined by SDS-PAGE.
Reconstitution Instructions:
Concentration of Human F-box / WD repeat-containing protein 7 Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant Human F-box / WD repeat-containing protein 7 Protein General Information | |
|---|---|
| Alternate Names | |
| FBX30; hCdc4; FBW6; hAgo; AGO; CDC4; FBW7; FBXO30; FBXW6; SEL-10; SEL10 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | FBXW7 |
| Entrez Gene ID | 55294 |
| Ensemble Gene ID | ENSG00000109670 |
| RefSeq Protein Accession(s) | NP_001013433.1 |
| RefSeq mRNA Accession(s) | NM_001013415.1, NM_018315.4, NM_033632.3, XM_011532083.1, XM_011532084.1, XM_011532085.1, XM_017008362.1 |
| UniProt ID(s) | Q969H0 |
| UniGene ID(s) | Hs.561245 |
| HGNC ID(s) | HGNC:16712 |
| COSMIC ID Link(s) | FBXW7 |
| KEGG Gene ID(s) | hsa:55294 |
| PharmGKB ID(s) | PA28054 |
| General Description of Recombinant Human F-box / WD repeat-containing protein 7 Protein. | |
| Substrate recognition component of an SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Recognizes and binds phosphorylated sites/phosphodegrons within target proteins and thereafter bring them to the SCF complex for ubiquitination (PubMed:17434132). Identified substrates include cyclin-E (CCNE1 or CCNE2), JUN, MYC, NOTCH1 released notch intracellular domain (NICD), and probably PSEN1 (PubMed:11565034, PubMed:12354302, PubMed:11585921, PubMed:15103331, PubMed:14739463, PubMed:17558397, PubMed:17873522, PubMed:22608923). Acts as a negative regulator of JNK signaling by binding to phosphorylated JUN and promoting its ubiquitination and subsequent degradation (PubMed:14739463). | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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