PDF DatasheetHuman Mitochondrial cardiolipin hydrolase Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant Mitochondrial cardiolipin hydrolase based upon sequence from: Human
Host: QP7879 protein expressed in E. coli.
Tag: His-SUMO
Protein Construction: A DNA sequence encoding the Homo sapiens (Human) Mitochondrial cardiolipin hydrolase, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Application Notes: Please contact us for application specific information for QP7879.
Bioactivity Data: Untested
Full Length? Full Length
Expression Region: Met1 – Thr252
Amino Acid Sequence: MGRLSWQVAA AAAVGLALTL EALPWVLRWL RSRRRRPRRE ALFFPSQVTC TEALLRAPGA ELAELPEGCP CGLPHGESAL SRLLRALLAA RASLDLCLFA FSSPQLGRAV QLLHQRGVRV RVVTDCDYMA LNGSQIGLLR KAGIQVRHDQ DPGYMHHKFA IVDKRVLITG SLNWTTQAIQ NNRENVLITE DDEYVRLFLE EFERIWEQFN PTKYTFFPPK KSHGSCAPPV SRAGGRLLSW HRTCGTSSES QT
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions:
Concentration of Human Mitochondrial cardiolipin hydrolase Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant Human Mitochondrial cardiolipin hydrolase Protein General Information | |
|---|---|
| Alternate Names | |
| ZUC | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | PLD6 |
| Entrez Gene ID | 201164 |
| Ensemble Gene ID | ENSG00000179598 |
| RefSeq Protein Accession(s) | NP_849158.2 |
| RefSeq mRNA Accession(s) | NM_178836.3 |
| UniProt ID(s) | Q8N2A8 |
| UniGene ID(s) | Hs.729079 |
| HGNC ID(s) | HGNC:30447 |
| COSMIC ID Link(s) | PLD6 |
| KEGG Gene ID(s) | hsa:201164 |
| PharmGKB ID(s) | PA164724625 |
| General Description of Recombinant Human Mitochondrial cardiolipin hydrolase Protein. | |
| Regulates mitochondrial shape through facilitating mitochondrial fusion. During spermatogenesis, plays a critical role in PIWI-interacting RNA (piRNA) biogenesis (By similarity). piRNAs provide essential protection against the activity of mobile genetic elements. piRNA-mediated transposon silencing is thus critical for maintaining genome stability, in particular in germline cells when transposons are mobilized as a consequence of wide-spread genomic demethylation. Has been shown to be a backbone-non-specific, single strand-specific nuclease, cleaving either RNA or DNA substrates with similar affinity (By similarity). Produces 5′ phosphate and 3′ hydroxyl termini, suggesting it could directly participate in the processing of primary piRNA transcripts (By similarity). Has been proposed to act as a cardiolipin hydrolase to generate phosphatidic acid at mitochondrial surface. Although it cannot be excluded that it can act as a phospholipase in some circumstances, it should be noted that cardiolipin hydrolase activity is either undetectable in vitro, or very low (PubMed:21397848). In addition, cardiolipin is almost exclusively found on the inner mitochondrial membrane, while PLD6 localizes to the outer mitochondrial membrane, facing the cytosol. | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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