PDF DatasheetHuman UBE2W Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant UBE2W based upon sequence from: Human
Host: QP7913 protein expressed in E. coli.
Tag: His-SUMO
Protein Construction: A DNA sequence encoding the Homo sapiens (Human) UBE2W, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Application Notes: Please contact us for application specific information for QP7913.
Bioactivity Data: Untested
Full Length? Full Length
Expression Region: Met1 – Cys151
Amino Acid Sequence: MASMQKRLQK ELLALQNDPP PGMTLNEKSV QNSITQWIVD MEGAPGTLYE GEKFQLLFKF SSRYPFDSPQ VMFTGENIPV HPHVYSNGHI CLSILTEDWS PALSVQSVCL SIISMLSSCK EKRRPPDNSF YVRTCNKNPK KTKWWYHDDT C
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions:
Concentration of Human UBE2W Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant Human UBE2W Protein General Information | |
|---|---|
| Curated Database and Bioinformatic Data | |
| Gene Symbol | UBE2W |
| Entrez Gene ID | 55284 |
| Ensemble Gene ID | ENSG00000104343 |
| RefSeq Protein Accession(s) | NP_001001481.2 |
| RefSeq mRNA Accession(s) | NM_001001481.2, NM_001271015.1, NM_018299.4 |
| UniProt ID(s) | Q96B02 |
| UniGene ID(s) | Hs.128841 |
| HGNC ID(s) | HGNC:25616 |
| COSMIC ID Link(s) | UBE2W |
| KEGG Gene ID(s) | hsa:55284 |
| PharmGKB ID(s) | PA142670657 |
| General Description of Recombinant Human UBE2W Protein. | |
| Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes monoubiquitination. Involved in degradation of misfolded chaperone substrates by mediating monoubiquitination of STUB1/CHIP, leading to recruitment of ATXN3 to monoubiquitinated STUB1/CHIP, and restriction of the length of ubiquitin chain attached to STUB1/CHIP substrates by ATXN3. After UV irradiation, but not after mitomycin-C (MMC) treatment, acts as a specific E2 ubiquitin-conjugating enzyme for the Fanconi ania complex by associating with E3 ubiquitin-protein ligase FANCL and catalyzing monoubiquitination of FANCD2, a key step in the DNA damage pathway. In vitro catalyzes ‘Lys-11’-linked polyubiquitination. Transfers ubiquitin in complex with RING/U-box type E3s that do not have active site cysteine residues to form thioester bonds with ubiquitin, and preferentially ubiquitinates the N-terminus of substrates, such as ATXN3, STUB1 and SUMO2. | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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