PDF DatasheetHuman NEIL1 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant NEIL1 based upon sequence from: Human
Host: QP7940 protein expressed in E. coli.
Tag: His-SUMO
Protein Construction: A DNA sequence encoding the Homo sapiens (Human) NEIL1, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Application Notes: Please contact us for application specific information for QP7940.
Bioactivity Data: Untested
Full Length? Full Length
Expression Region: Pro2 – Ser390
Amino Acid Sequence: PEGPELHLAS QFVNEACRAL VFGGCVEKSS VSRNPEVPFE SSAYRISASA RGKELRLILS PLPGAQPQQE PLALVFRFGM SGSFQLVPRE ELPRHAHLRF YTAPPGPRLA LCFVDIRRFG RWDLGGKWQP GRGPCVLQEY QQFRENVLRN LADKAFDRPI CEALLDQRFF NGIGNYLRAE ILYRLKIPPF EKARSVLEAL QQHRPSPELT LSQKIRTKLQ NPDLLELCHS VPKEVVQLGG KGYGSESGEE DFAAFRAWLR CYGMPGMSSL QDRHGRTIWF QGDPGPLAPK GRKSRKKKSK ATQLSPEDRV EDALPPSKAP SRTRRAKRDL PKRTATQRPE GTSLQQDPEA PTVPKKGRRK GRQAASGHCR PRKVKADIPS LEPEGTSAS
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions:
Concentration of Human NEIL1 Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant Human NEIL1 Protein General Information | |
|---|---|
| Alternate Names | |
| NEIL1; NEI1; FPG1; hFPG1 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | NEIL1 |
| Entrez Gene ID | 79661 |
| Ensemble Gene ID | ENSG00000140398 |
| RefSeq Protein Accession(s) | NP_001243481.1 |
| RefSeq mRNA Accession(s) | NM_001256552.1, NM_024608.3, XM_005254659.4, XM_006720680.1, XM_006720681.1, XM_011522002.1, XM_011522003.2, XM_011522004.2 |
| UniProt ID(s) | Q96FI4 |
| UniGene ID(s) | Hs.512732 |
| HGNC ID(s) | HGNC:18448 |
| COSMIC ID Link(s) | NEIL1 |
| KEGG Gene ID(s) | hsa:79661 |
| PharmGKB ID(s) | PA38334 |
| General Description of Recombinant Human NEIL1 Protein. | |
| Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and roves damaged bases. Has a preference for oxidized pyrimidines, such as thymine glycol, formamidopyrimidine (Fapy) and 5-hydroxyuracil. Has marginal activity towards 8-oxoguanine. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the roved base with both 3′- and 5′-phosphates. Has DNA glycosylase/lyase activity towards mismatched uracil and thymine, in particular in U:C and T:C mismatches. Specifically binds 5-hydroxymethylcytosine (5hmC), suggesting that it acts as a specific reader of 5hmC. | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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