PDF DatasheetHuman CHCHD4 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant CHCHD4 based upon sequence from: Human
Host: QP7968 protein expressed in E. coli.
Tag: His-SUMO
Protein Construction: A DNA sequence encoding the Homo sapiens (Human) CHCHD4, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Application Notes: Please contact us for application specific information for QP7968.
Bioactivity Data: Untested
Full Length? Full Length
Expression Region: Met1 – Ser142
Amino Acid Sequence: MSYCRQEGKD RIIFVTKEDH ETPSSAELVA DDPNDPYEEH GLILPNGNIN WNCPCLGGMA SGPCGEQFKS AFSCFHYSTE EIKGSDCVDQ FRAMQECMQK YPDLYPQEDE DEEEEREKKP AEQAEETAPI EATATKEEEG SS
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions:
Concentration of Human CHCHD4 Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant Human CHCHD4 Protein General Information | |
|---|---|
| Alternate Names | |
| MIA40; TIMM40; Mitochondrial intermembrane space import and assembly protein | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | CHCHD4 |
| Entrez Gene ID | 131474 |
| Ensemble Gene ID | ENSG00000163528 |
| RefSeq Protein Accession(s) | NP_001091972.1 |
| RefSeq mRNA Accession(s) | NM_001098502.1, NM_144636.2 |
| UniProt ID(s) | Q8N4Q1 |
| UniGene ID(s) | Hs.517815 |
| HGNC ID(s) | HGNC:26467 |
| COSMIC ID Link(s) | CHCHD4 |
| KEGG Gene ID(s) | hsa:131474 |
| PharmGKB ID(s) | PA134990707 |
| General Description of Recombinant Human CHCHD4 Protein. | |
| Functions as chaperone and catalyzes the formation of disulfide bonds in substrate proteins, such as COX17. Required for the import and folding of small cysteine-containing proteins (small Tim) in the mitochondrial intermbrane space (IMS). Precursor proteins to be imported into the IMS are translocated in their reduced form into the mitochondria. The oxidized form of CHCHD4/MIA40 forms a transient intermolecular disulfide bridge with the reduced precursor protein, resulting in oxidation of the precursor protein that now contains an intramolecular disulfide bond and is able to undergo folding in the IMS. Reduced CHCHD4/MIA40 is then reoxidized by GFER/ERV1 via a disulfide relay syst. | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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