PDF DatasheetHuman Crossover junction endonuclease EME1 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant Crossover junction endonuclease EME1 based upon sequence from: Human
Isoform: Isoform 2
Host: QP8003 protein expressed in E. coli.
Tag: His-SUMO
Protein Construction: A DNA sequence encoding the Homo sapiens (Human) Crossover junction endonuclease EME1, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Application Notes: Please contact us for application specific information for QP8003.
Bioactivity Data: Untested
Full Length? Full Length of Isoform 2
Expression Region: Met1 – Asp583
Amino Acid Sequence: MALKKSSPSL DSGDSDSEEL PTFAFLKKEP SSTKRRQPER EEKIVVVDIS DCEASCPPAP ELFSPPVPEI AETVTQTQPV RLLSSESEDE EEFIPLAQRL TCKFLTHKQL SPEDSSSPVK SVLDHQNNEG ASCDWKKPFP KIPEVPLHDT PERSAADNKD LILDPCCQLP AYLSTCPGQS SSLAVTKTNS DILPPQKKTK PSQKVQGRGS HGCRQQRQAR QKESTLRRQE RKNAALVTRM KAQRPEECLK HIIVVLDPVL LQMEGGGQLL GALQTMECRC VIEAQAVPCS VTWRRRAGPS EDREDWVEEP TVLVLLRAEA FVSMIDNGKQ GSLDSTMKGK ETLQGFVTDI TAKTAGKALS LVIVDQEKCF SLELLFFDFL PCTSAQNPPR RGKQGANKQT KKQQQRQPEA SIGSMVSRVD AEEALVDLQL HTEAQAQIVQ SWKELADFTC AFTKAVAEAP FKKLRDETTF SFCLESDWAG GVKVDLAGRG LALVWRRQIQ QLNRVSLEMA SAVVNAYPSP QLLVQAYQQC FSDKERQNLL ADIQVRRGEG VTSTSRRIGP ELSRRIYLQM TTLQPHLSLD SAD
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions:
Concentration of Human Crossover junction endonuclease EME1 Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant Human Crossover junction endonuclease EME1 Protein General Information | |
|---|---|
| Alternate Names | |
| MMS4L; SLX2A | |
| Molecular Weight | |
| Isoform 2 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | EME1 |
| Entrez Gene ID | 146956 |
| Ensemble Gene ID | ENSG00000154920 |
| RefSeq Protein Accession(s) | NP_001159603.1 |
| RefSeq mRNA Accession(s) | NM_001166131.1, NM_152463.2 |
| UniProt ID(s) | Q96AY2 |
| UniGene ID(s) | Hs.514330 |
| HGNC ID(s) | HGNC:24965 |
| COSMIC ID Link(s) | EME1 |
| KEGG Gene ID(s) | hsa:146956 |
| PharmGKB ID(s) | PA134904115 |
| General Description of Recombinant Human Crossover junction endonuclease EME1 Protein. | |
| Interacts with MUS81 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5′-end at the branch nick. Typical substrates include 3′-flap structures, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication forks. | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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