PDF DatasheetHuman MAPKAP1 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant MAPKAP1 based upon sequence from: Human
Host: QP8011 protein expressed in E. coli.
Tag: His-SUMO
Protein Construction: A DNA sequence encoding the Homo sapiens (Human) MAPKAP1, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Application Notes: Please contact us for application specific information for QP8011.
Bioactivity Data: Untested
Full Length? Full Length
Expression Region: Ala2 – Gln522
Amino Acid Sequence: AFLDNPTIIL AHIRQSHVTS DDTGMCEMVL IDHDVDLEKI HPPSMPGDSG SEIQGSNGET QGYVYAQSVD ITSSWDFGIR RRSNTAQRLE RLRKERQNQI KCKNIQWKER NSKQSAQELK SLFEKKSLKE KPPISGKQSI LSVRLEQCPL QLNNPFNEYS KFDGKGHVGT TATKKIDVYL PLHSSQDRLL PMTVVTMASA RVQDLIGLIC WQYTSEGREP KLNDNVSAYC LHIAEDDGEV DTDFPPLDSN EPIHKFGFST LALVEKYSSP GLTSKESLFV RINAAHGFSL IQVDNTKVTM KEILLKAVKR RKGSQKVSGP QYRLEKQSEP NVAVDLDSTL ESQSAWEFCL VRENSSRADG VFEEDSQIDI ATVQDMLSSH HYKSFKVSMI HRLRFTTDVQ LGISGDKVEI DPVTNQKAST KFWIKQKPIS IDSDLLCACD LAEEKSPSHA IFKLTYLSNH DYKHLYFESD AATVNEIVLK VNYILESRAS TARADYFAQK QRKLNRRTSF SFQKEKKSGQ Q
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions:
Concentration of Human MAPKAP1 Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant Human MAPKAP1 Protein General Information | |
|---|---|
| Alternate Names | |
| MIP1; SIN1b; SIN1; SIN1g; JC310; Target of rapamycin complex 2 subunit MAPKA | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | MAPKAP1 |
| Entrez Gene ID | 79109 |
| Ensemble Gene ID | ENSG00000119487 |
| RefSeq Protein Accession(s) | NP_001006618.1 |
| RefSeq mRNA Accession(s) | NM_001006617.1, NM_001006618.1, NM_001006619.1, NM_001006620.1, NM_001006621.1, NM_024117.3, XM_011519010.2, XM_017015126.1, XM_017015127.1 |
| UniProt ID(s) | Q9BPZ7 |
| UniGene ID(s) | Hs.495138 |
| HGNC ID(s) | HGNC:18752 |
| COSMIC ID Link(s) | MAPKAP1 |
| KEGG Gene ID(s) | hsa:79109 |
| PharmGKB ID(s) | PA38674 |
| General Description of Recombinant Human MAPKAP1 Protein. | |
| Subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 ‘Ser-473’ phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on ‘Thr-308’ by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at ‘Ser-422’. mTORC2 also modulates the phosphorylation of PRKCA on ‘Ser-657’. Within mTORC2, MAPKAP1 is required for complex formation and mTORC2 kinase activity. MAPKAP1 inhibits MAP3K2 by preventing its dimerization and autophosphorylation. Inhibits HRAS and KRAS signaling. Enhances osmotic stress-induced phosphorylation of ATF2 and ATF2-mediated transcription. Involved in ciliogenesis, regulates cilia length through its interaction with CCDC28B independently of mTORC2 complex. | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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