PDF DatasheetHuman E3 ubiquitin-protein ligase SMURF2 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant E3 ubiquitin-protein ligase SMURF2 based upon sequence from: Human
Host: QP8114 protein expressed in E.coli, Yeast..
Tag: GST
Protein Construction: A DNA sequence encoding the Homo sapiens (Human) E3 ubiquitin-protein ligase SMURF2, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Application Notes: Please contact us for application specific information for QP8114.
Bioactivity Data: Untested
Full Length? Full Length
Expression Region: Met1 – Glu748
Amino Acid Sequence: MSNPGGRRNG PVKLRLTVLC AKNLVKKDFF RLPDPFAKVV VDGSGQCHST DTVKNTLDPK WNQHYDLYIG KSDSVTISVW NHKKIHKKQG AGFLGCVRLL SNAINRLKDT GYQRLDLCKL GPNDNDTVRG QIVVSLQSRD RIGTGGQVVD CSRLFDNDLP DGWEERRTAS GRIQYLNHIT RTTQWERPTR PASEYSSPGR PLSCFVDENT PISGTNGATC GQSSDPRLAE RRVRSQRHRN YMSRTHLHTP PDLPEGYEQR TTQQGQVYFL HTQTGVSTWH DPRVPRDLSN INCEELGPLP PGWEIRNTAT GRVYFVDHNN RTTQFTDPRL SANLHLVLNR QNQLKDQQQQ QVVSLCPDDT ECLTVPRYKR DLVQKLKILR QELSQQQPQA GHCRIEVSRE EIFEESYRQV MKMRPKDLWK RLMIKFRGEE GLDYGGVARE WLYLLSHEML NPYYGLFQYS RDDIYTLQIN PDSAVNPEHL SYFHFVGRIM GMAVFHGHYI DGGFTLPFYK QLLGKSITLD DMELVDPDLH NSLVWILEND ITGVLDHTFC VEHNAYGEII QHELKPNGKS IPVNEENKKE YVRLYVNWRF LRGIEAQFLA LQKGFNEVIP QHLLKTFDEK ELELIICGLG KIDVNDWKVN TRLKHCTPDS NIVKWFWKAV EFFDEERRAR LLQFVTGSSR VPLQGFKALQ GAAGPRLFTI HQIDACTNNL PKAHTCFNRI DIPPYESYEK LYEKLLTAIE ETCGFAVE
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions:
Concentration of Human E3 ubiquitin-protein ligase SMURF2 Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant Human E3 ubiquitin-protein ligase SMURF2 Protein General Information | |
|---|---|
| Curated Database and Bioinformatic Data | |
| Gene Symbol | SMURF2 |
| Entrez Gene ID | 64750 |
| Ensemble Gene ID | ENSG00000108854 |
| RefSeq Protein Accession(s) | NP_073576.1 |
| RefSeq mRNA Accession(s) | NM_022739.3 |
| UniProt ID(s) | Q9HAU4 |
| UniGene ID(s) | Hs.515011 |
| HGNC ID(s) | HGNC:16809 |
| COSMIC ID Link(s) | SMURF2 |
| KEGG Gene ID(s) | hsa:64750 |
| PharmGKB ID(s) | PA134985524 |
| General Description of Recombinant Human E3 ubiquitin-protein ligase SMURF2 Protein. | |
| E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Interacts with SMAD1 and SMAD7 in order to trigger their ubiquitination and proteasome-dependent degradation. In addition, interaction with SMAD7 activates autocatalytic degradation, which is prevented by interaction with SCYE1. Forms a stable complex with the TGF-beta receptor-mediated phosphorylated SMAD2 and SMAD3. In this way, SMAD2 may recruit substrates, such as SNON, for ubiquitin-mediated degradation. Enhances the inhibitory activity of SMAD7 and reduces the transcriptional activity of SMAD2. Coexpression of SMURF2 with SMAD1 results in considerable decrease in steady-state level of SMAD1 protein and a smaller decrease of SMAD2 level. | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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