PDF DatasheetHuman Tripartite motif-containing protein 5 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant Tripartite motif-containing protein 5 based upon sequence from: Human
Host: QP8134 protein expressed in E.coli, Invitro-E.coli..
Tag: His
Protein Construction: A DNA sequence encoding the Homo sapiens (Human) Tripartite motif-containing protein 5, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Application Notes: Please contact us for application specific information for QP8134.
Bioactivity Data: Untested
Full Length? Full Length
Expression Region: Met1 – Ser493
Amino Acid Sequence: MASGILVNVK EEVTCPICLE LLTQPLSLDC GHSFCQACLT ANHKKSMLDK GESSCPVCRI SYQPENIRPN RHVANIVEKL REVKLSPEGQ KVDHCARHGE KLLLFCQEDG KVICWLCERS QEHRGHHTFL TEEVAREYQV KLQAALEMLR QKQQEAEELE ADIREEKASW KTQIQYDKTN VLADFEQLRD ILDWEESNEL QNLEKEEEDI LKSLTNSETE MVQQTQSLRE LISDLEHRLQ GSVMELLQGV DGVIKRTENV TLKKPETFPK NQRRVFRAPD LKGMLEVFRE LTDVRRYWVD VTVAPNNISC AVISEDKRQV SSPKPQIIYG ARGTRYQTFV NFNYCTGILG SQSITSGKHY WEVDVSKKTA WILGVCAGFQ PDAMCNIEKN ENYQPKYGYW VIGLEEGVKC SAFQDSSFHT PSVPFIVPLS VIICPDRVGV FLDYEACTVS FFNITNHGFL IYKFSHCSFS QPVFPYLNPR KCGVPMTLCS PSS
Purity: Greater than 85% as determined by SDS-PAGE.
Reconstitution Instructions:
Concentration of Human Tripartite motif-containing protein 5 Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant Human Tripartite motif-containing protein 5 Protein General Information | |
|---|---|
| Alternate Names | |
| TRIM5alpha; RNF88 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | TRIM5 |
| Entrez Gene ID | 85363 |
| Ensemble Gene ID | ENSG00000132256 |
| RefSeq Protein Accession(s) | NP_149023.2 |
| RefSeq mRNA Accession(s) | NM_033034.2, NM_033092.2, NM_033093.2, XM_005253183.2, XM_005253184.2, XM_011520427.1 |
| UniProt ID(s) | Q9C035 |
| UniGene ID(s) | Hs.125300 |
| HGNC ID(s) | HGNC:16276 |
| COSMIC ID Link(s) | TRIM5 |
| KEGG Gene ID(s) | hsa:85363 |
| PharmGKB ID(s) | PA38109 |
| General Description of Recombinant Human Tripartite motif-containing protein 5 Protein. | |
| Capsid-specific restriction factor that prevents infection from non-host-adapted retroviruses. Blocks viral replication early in the life cycle, after viral entry but before reverse transcription. In addition to acting as a capsid-specific restriction factor, also acts as a pattern recognition receptor that activates innate immune signaling in response to the retroviral capsid lattice. Binding to the viral capsid triggers its E3 ubiquitin ligase activity, and in concert with the heterodimeric ubiquitin conjugating enzyme complex UBE2V1-UBE2N (also known as UBC13-UEV1A complex) generates ‘Lys-63’-linked polyubiquitin chains, which in turn are catalysts in the autophosphorylation of the MAP3K7/TAK1 complex (includes TAK1, TAB2, and TAB3). Activation of the MAP3K7/TAK1 complex by autophosphorylation results in the induction and expression of NF-kappa-B and MAPK-responsive inflammatory genes, thereby leading to an innate immune response in the infected cell. Restricts infection by N-tropic murine leukemia virus (N-MLV), equine infectious anemia virus (EIAV), simian immunodeficiency virus of macaques (SIVmac), feline immunodeficiency virus (FIV), and bovine immunodeficiency virus (BIV). Plays a role in regulating autophagy through activation of autophagy regulator BECN1 by causing its dissociation from its inhibitors BCL2 and TAB2. Also plays a role in autophagy by acting as a selective autophagy receptor which recognizes and targets HIV-1 capsid protein p24 for autophagic destruction. | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
There are no reviews yet.