PDF DatasheetHuman PRKAG2 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant PRKAG2 based upon sequence from: Human
Isoform: Isoform B
Host: QP8207 protein expressed in E. coli.
Tag: His
Protein Construction: A DNA sequence encoding the Homo sapiens (Human) PRKAG2, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Application Notes: Please contact us for application specific information for QP8207.
Bioactivity Data: Untested
Full Length? Full Length of Isoform B
Expression Region: Met1 – Glu328
Amino Acid Sequence: MLEKLEFEDE AVEDSESGVY MRFMRSHKCY DIVPTSSKLV VFDTTLQVKK AFFALVANGV RAAPLWESKK QSFVGMLTIT DFINILHRYY KSPMVQIYEL EEHKIETWRE LYLQETFKPL VNISPDASLF DAVYSLIKNK IHRLPVIDPI SGNALYILTH KRILKFLQLF MSDMPKPAFM KQNLDELGIG TYHNIAFIHP DTPIIKALNI FVERRISALP VVDESGKVVD IYSKFDVINL AAEKTYNNLD ITVTQALQHR SQYFEGVVKC NKLEILETIV DRIVRAEVHR LVVVNEADSI VGIISLSDIL QALILTPAGA KQKETETE
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions:
Concentration of Human PRKAG2 Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant Human PRKAG2 Protein General Information | |
|---|---|
| Alternate Names | |
| CMH6; H91620p; WPWS; AAKG; AAKG2; 5′-AMP-activated protein kinase subunit gamma | |
| Molecular Weight | |
| Isoform B | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | PRKAG2 |
| Entrez Gene ID | 51422 |
| Ensemble Gene ID | ENSG00000106617 |
| RefSeq Protein Accession(s) | NP_001035723.1 |
| RefSeq mRNA Accession(s) | NM_001040633.1, NM_001304527.1, NM_001304531.1, NM_016203.3, NM_024429.1, XM_017012270.1 |
| UniProt ID(s) | Q9UGJ0 |
| UniGene ID(s) | Hs.647072 |
| HGNC ID(s) | HGNC:9386 |
| COSMIC ID Link(s) | PRKAG2 |
| KEGG Gene ID(s) | hsa:51422 |
| PharmGKB ID(s) | PA33752 |
| General Description of Recombinant Human PRKAG2 Protein. | |
| AMP/ATP-binding subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Gamma non-catalytic subunit mediates binding to AMP, ADP and ATP, leading to activate or inhibit AMPK: AMP-binding results in allosteric activation of alpha catalytic subunit (PRKAA1 or PRKAA2) both by inducing phosphorylation and preventing dephosphorylation of catalytic subunits. ADP also stimulates phosphorylation, without stimulating already phosphorylated catalytic subunit. ATP promotes dephosphorylation of catalytic subunit, rendering the AMPK enzyme inactive. | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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