PDF DatasheetHuman ISCU, mitochondrial Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant ISCU, mitochondrial based upon sequence from: Human
Host: QP8214 protein expressed in E. coli.
Tag: GST
Protein Construction: A DNA sequence encoding the Homo sapiens (Human) ISCU, mitochondrial, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Application Notes: Please contact us for application specific information for QP8214.
Bioactivity Data: Untested
Full Length? Full Length
Expression Region: Tyr1 – Lys167
Amino Acid Sequence: YHKKVVDHYE NPRNVGSLDK TSKNVGTGLV GAPACGDVMK LQIQVDEKGK IVDARFKTFG CGSAIASSSL ATEWVKGKTV EEALTIKNTD IAKELCLPPV KLHCSMLAED AIKAALADYK LKQEPKKGEA EKK
Purity: Greater than 80% as determined by SDS-PAGE.
Reconstitution Instructions:
Concentration of Human ISCU, mitochondrial Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant Human ISCU, mitochondrial Protein General Information | |
|---|---|
| Alternate Names | |
| 2310020H20Rik; HML; NIFU; ISU2; NIFUN; hnifU; Iron-sulfur cluster assembly enzyme ISCU, mitochondri | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | ISCU |
| Entrez Gene ID | 23479 |
| Ensemble Gene ID | ENSG00000136003 |
| RefSeq Protein Accession(s) | NP_001306971.1 |
| RefSeq mRNA Accession(s) | NM_001320042.1, NM_014301.4, NM_213595.3 |
| UniProt ID(s) | Q9H1K1 |
| UniGene ID(s) | Hs.615131 |
| HGNC ID(s) | HGNC:29882 |
| COSMIC ID Link(s) | ISCU |
| KEGG Gene ID(s) | hsa:23479 |
| PharmGKB ID(s) | PA162392328 |
| General Description of Recombinant Human ISCU, mitochondrial Protein. | |
| Scaffold protein for the de novo synthesis of iron-sulfur (Fe-S) clusters within mitochondria, which is required for maturation of both mitochondrial and cytoplasmic [2Fe-2S] and [4Fe-4S] proteins. First, a [2Fe-2S] cluster is transiently assembled on the scaffold protein ISCU. In a second step, the cluster is released from ISCU, transferred to a glutaredoxin GLRX5, followed by the formation of mitochondrial [2Fe-2S] proteins, the synthesis of [4Fe-4S] clusters and their target-specific insertion into the recipient apoproteins. Cluster assembly on ISCU depends on the function of the cysteine desulfurase complex NFS1-LYRM4/ISD11, which serves as the sulfur donor for cluster synthesis, the iron-binding protein frataxin as the putative iron donor, and the electron transfer chain comprised of ferredoxin reductase and ferredoxin, which receive their electrons from NADH | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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