PDF DatasheetHuman Charged multivesicular body protein 4b Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant Charged multivesicular body protein 4b based upon sequence from: Human
Host: QP8215 protein expressed in E. coli.
Tag: GST
Protein Construction: A DNA sequence encoding the Homo sapiens (Human) Charged multivesicular body protein 4b, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Application Notes: Please contact us for application specific information for QP8215.
Bioactivity Data: Untested
Full Length? Full Length
Expression Region: Ser1 – Met224
Amino Acid Sequence: SVFGKLFGAG GGKAGKGGPT PQEAIQRLRD TEEMLSKKQE FLEKKIEQEL TAAKKHGTKN KRAALQALKR KKRYEKQLAQ IDGTLSTIEF QREALENANT NTEVLKNMGY AAKAMKAAHD NMDIDKVDEL MQDIADQQEL AEEISTAISK PVGFGEEFDE DELMAELEEL EQEELDKNLL EISGPETVPL PNVPSIALPS KPAKKKEEED DDMKELENWA GSM
Purity: Greater than 80% as determined by SDS-PAGE.
Reconstitution Instructions:
Concentration of Human Charged multivesicular body protein 4b Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant Human Charged multivesicular body protein 4b Protein General Information | |
|---|---|
| Alternate Names | |
| SNF7; CTRCT31; dJ553F4.4; Vps32-2; C20orf178; CHMP4A; Shax1; CTPP3; SNF7-2; VPS32B | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | CHMP4B |
| Entrez Gene ID | 128866 |
| Ensemble Gene ID | ENSG00000101421 |
| RefSeq Protein Accession(s) | NP_789782.1 |
| RefSeq mRNA Accession(s) | NM_176812.4 |
| UniProt ID(s) | Q9H444 |
| UniGene ID(s) | Hs.472471 |
| HGNC ID(s) | HGNC:16171 |
| COSMIC ID Link(s) | CHMP4B |
| KEGG Gene ID(s) | hsa:128866 |
| PharmGKB ID(s) | PA25721 |
| General Description of Recombinant Human Charged multivesicular body protein 4b Protein. | |
| Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis. Together with SPAST, the ESCRT-III complex promotes nuclear envelope sealing and mitotic spindle disassembly during late anaphase. Plays a role in the endosomal sorting pathway. ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. When overexpressed, membrane-assembled circular arrays of CHMP4B filaments can promote or stabilize negative curvature and outward budding. CHMP4A/B/C are required for the exosomal release of SDCBP, CD63 and syndecan The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the budding of enveloped viruses (HIV-1 and other lentiviruses). Via its interaction with PDCD6IP involved in HIV-1 p6- and p9-dependent virus release. |
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Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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