PDF DatasheetHuman AGO2 / Argonaute 2 / EIF2C2 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant AGO2 / Argonaute 2 / EIF2C2 based upon sequence from: Human
Host: QP8243 protein expressed in E.coli, Yeast..
Tag: His
Protein Construction: A DNA sequence encoding the Homo sapiens (Human) AGO2 / Argonaute 2 / EIF2C2, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Application Notes: Please contact us for application specific information for QP8243.
Bioactivity Data: Untested
Full Length? Partial (see sequence information for more details).
Expression Region: Leu517 – Val818
Amino Acid Sequence: LVVVILPGKT PVYAEVKRVG DTVLGMATQC VQMKNVQRTT PQTLSNLCLK INVKLGGVNN ILLPQGRPPV FQQPVIFLGA DVTHPPAGDG KKPSIAAVVG SMDAHPNRYC ATVRVQQHRQ EIIQDLAAMV RELLIQFYKS TRFKPTRIIF YRDGVSEGQF QQVLHHELLA IREACIKLEK DYQPGITFIV VQKRHHTRLF CTDKNERVGK SGNIPAGTTV DTKITHPTEF DFYLCSHAGI QGTSRPSHYH VLWDDNRFSS DELQILTYQL CHTYVRCTRS VSIPAPAYYA HLVAFRARYH LV
Purity: Greater than 80% as determined by SDS-PAGE.
Reconstitution Instructions:
Concentration of Human AGO2 / Argonaute 2 / EIF2C2 Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant Human AGO2 / Argonaute 2 / EIF2C2 Protein General Information | |
|---|---|
| Alternate Names | |
| AGO2; Argonaute 2; EIF2C2; AI225898; AL022874; Eif2c2; Gm10365; 2310051F07Rik; Gerp95; mKIAA4215; 1110029L17Rik; AW546247; ENSMUSG00000072493 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | AGO2 |
| Entrez Gene ID | 27161 |
| Ensemble Gene ID | ENSG00000123908 |
| RefSeq Protein Accession(s) | NP_001158095.1 |
| RefSeq mRNA Accession(s) | NM_001164623.2, NM_012154.4 |
| UniProt ID(s) | Q9UKV8 |
| UniGene ID(s) | Hs.660189 |
| HGNC ID(s) | HGNC:3263 |
| KEGG Gene ID(s) | hsa:27161 |
| PharmGKB ID(s) | PA27694 |
| General Description of Recombinant Human AGO2 / Argonaute 2 / EIF2C2 Protein. | |
| Required for RNA-mediated gene silencing (RNAi) by the RNA-induced silencing complex (RISC). The ‘minimal RISC’ appears to include AGO2 bound to a short guide RNA such as a microRNA (miRNA) or short interfering RNA (siRNA). These guide RNAs direct RISC to complentary mRNAs that are targets for RISC-mediated gene silencing. The precise mechanism of gene silencing depends on the degree of complentarity between the miRNA or siRNA and its target. Binding of RISC to a perfectly complentary mRNA generally results in silencing due to endonucleolytic cleavage of the mRNA specifically by AGO2. Binding of RISC to a partially complentary mRNA results in silencing through inhibition of translation, and this is independent of endonuclease activity. May inhibit translation initiation by binding to the 7-methylguanosine cap, thereby preventing the recruitment of the translation initiation factor eIF4-E. May also inhibit translation initiation via interaction with EIF6, which itself binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit. The inhibition of translational initiation leads to the accumulation of the affected mRNA in Cytoplasmic domain processing bodies (P-bodies), where mRNA degradation may subsequently occur. In some cases RISC-mediated translational repression is also observed for miRNAs that perfectly match the 3′ untranslated region (3′-UTR). Can also up-regulate the translation of specific mRNAs under certain growth conditions. Binds to the AU elent of the 3′-UTR of the TNF (TNF-alpha) mRNA and up-regulates translation under conditions of serum starvation. Also required for transcriptional gene silencing (TGS), in which short RNAs known as antigene RNAs or agRNAs direct the transcriptional repression of complentary promoter regions | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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