PDF DatasheetHuman F-box / LRR-repeat protein 2 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant F-box / LRR-repeat protein 2 based upon sequence from: Human
Host: QP8251 protein expressed in E. coli.
Tag: His-SUMO
Protein Construction: A DNA sequence encoding the Homo sapiens (Human) F-box/LRR-repeat protein 2, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Application Notes: Please contact us for application specific information for QP8251.
Bioactivity Data: Untested
Full Length? Full Length
Expression Region: Met1 – Leu423
Amino Acid Sequence: MVFSNNDEGL INKKLPKELL LRIFSFLDIV TLCRCAQISK AWNILALDGS NWQRIDLFNF QTDVEGRVVE NISKRCGGFL RKLSLRGCIG VGDSSLKTFA QNCRNIEHLN LNGCTKITDS TCYSLSRFCS KLKHLDLTSC VSITNSSLKG ISEGCRNLEY LNLSWCDQIT KDGIEALVRG CRGLKALLLR GCTQLEDEAL KHIQNYCHEL VSLNLQSCSR ITDEGVVQIC RGCHRLQALC LSGCSNLTDA SLTALGLNCP RLQILEAARC SHLTDAGFTL LARNCHELEK MDLEECILIT DSTLIQLSIH CPKLQALSLS HCELITDDGI LHLSNSTCGH ERLRVLELDN CLLITDVALE HLENCRGLER LELYDCQQVT RAGIKRMRAQ LPHVKVHAYF APVTPPTAVA GSGQRLCRCC VIL
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions:
Concentration of Human F-box / LRR-repeat protein 2 Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant Human F-box / LRR-repeat protein 2 Protein General Information | |
|---|---|
| Alternate Names | |
| FBL2; FBL3 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | FBXL2 |
| Entrez Gene ID | 25827 |
| Ensemble Gene ID | ENSG00000153558 |
| RefSeq Protein Accession(s) | NP_001165184.1 |
| RefSeq mRNA Accession(s) | NM_001171713.1, NM_012157.3 |
| UniProt ID(s) | Q9UKC9 |
| UniGene ID(s) | Hs.475872 |
| HGNC ID(s) | HGNC:13598 |
| COSMIC ID Link(s) | FBXL2 |
| KEGG Gene ID(s) | hsa:25827 |
| PharmGKB ID(s) | PA28021 |
| General Description of Recombinant Human F-box / LRR-repeat protein 2 Protein. | |
| Calcium-activated substrate recognition component of the SCF (SKP1-cullin-F-box protein) E3 ubiquitin-protein ligase complex, SCF(FBXL2), which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Unlike many F-box proteins, FBXL2 does not seem to target phosphodegron within its substrates but rather calmodulin-binding motifs and is thereby antagonized by calmodulin. This is the case for the cyclins CCND2 and CCND3 which polyubiquitination and subsequent degradation are inhibited by calmodulin. Through CCND2 and CCND3 degradation induces cell-cycle arrest in G0 (PubMed:22020328, PubMed:22323446). SCF(FBXL2) also mediates PIK3R2 ubiquitination and proteasomal degradation thereby regulating phosphatidylinositol 3-kinase signaling and autophagy (PubMed:23604317). PCYT1A monoubiquitination by SCF(FBXL2) and subsequent degradation regulates synthesis of phosphatidylcholine, which is utilized for formation of membranes and of pulmonary surfactant (By similarity). | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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