PDF DatasheetHuman cytosine-5 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant cytosine-5 based upon sequence from: Human
Host: QP8293 protein expressed in E. coli.
Tag: His
Protein Construction: A DNA sequence encoding the Homo sapiens (Human) cytosine-5, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Application Notes: Please contact us for application specific information for QP8293.
Bioactivity Data: Untested
Full Length? Partial (see sequence information for more details).
Expression Region: Lys680 – Phe902
Amino Acid Sequence: KIMYVGDVRS VTQKHIQEWG PFDLVIGGSP CNDLSIVNPA RKGLYEGTGR LFFEFYRLLH DARPKEGDDR PFFWLFENVV AMGVSDKRDI SRFLESNPVM IDAKEVSAAH RARYFWGNLP GMNRPLASTV NDKLELQECL EHGRIAKFSK VRTITTRSNS IKQGKDQHFP VFMNEKEDIL WCTEMERVFG FPVHYTDVSN MSRLARQRLL GRSWSVPVIR HLF
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions:
Concentration of Human cytosine-5 Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant Human cytosine-5 Protein General Information | |
|---|---|
| Alternate Names | |
| DNMT3A2; M.HsaIIIA; TBRS; DNA (cytosine-5)-methyltransferase | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | DNMT3A |
| Entrez Gene ID | 1788 |
| Ensemble Gene ID | ENSG00000119772 |
| RefSeq Protein Accession(s) | NP_001307821.1 |
| RefSeq mRNA Accession(s) | NM_001320892.1, NM_001320893.1, NM_022552.4, NM_153759.3, NM_175629.2, NM_175630.1, XM_005264175.4, XM_005264177.4, XM_011532667.2, XM_017003526.1, XM_017003527.1 |
| UniProt ID(s) | Q9Y6K1 |
| UniGene ID(s) | Hs.515840 |
| HGNC ID(s) | HGNC:2978 |
| COSMIC ID Link(s) | DNMT3A |
| KEGG Gene ID(s) | hsa:1788 |
| PharmGKB ID(s) | PA27445 |
| General Description of Recombinant Human cytosine-5 Protein. | |
| Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. Plays a role in paternal and maternal imprinting. Required for methylation of most imprinted loci in germ cells. Acts as a transcriptional corepressor for ZBTB18. Recruited to trimethylated ‘Lys-36’ of histone H3 (H3K36me3) sites. Can actively repress transcription through the recruitment of HDAC activity. | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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