PDF DatasheetHuman RuvB-like 2 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant RuvB-like 2 based upon sequence from: Human
Host: QP8295 protein expressed in E. coli.
Tag: His-SUMO
Protein Construction: A DNA sequence encoding the Homo sapiens (Human) RuvB-like 2, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Application Notes: Please contact us for application specific information for QP8295.
Bioactivity Data: Untested
Full Length? Full Length
Expression Region: Ala2 – Ser463
Amino Acid Sequence: ATVTATTKVP EIRDVTRIER IGAHSHIRGL GLDDALEPRQ ASQGMVGQLA ARRAAGVVLE MIREGKIAGR AVLIAGQPGT GKTAIAMGMA QALGPDTPFT AIAGSEIFSL EMSKTEALTQ AFRRSIGVRI KEETEIIEGE VVEIQIDRPA TGTGSKVGKL TLKTTEMETI YDLGTKMIES LTKDKVQAGD VITIDKATGK ISKLGRSFTR ARDYDAMGSQ TKFVQCPDGE LQKRKEVVHT VSLHEIDVIN SRTQGFLALF SGDTGEIKSE VREQINAKVA EWREEGKAEI IPGVLFIDEV HMLDIESFSF LNRALESDMA PVLIMATNRG ITRIRGTSYQ SPHGIPIDLL DRLLIVSTTP YSEKDTKQIL RIRCEEEDVE MSEDAYTVLT RIGLETSLRY AIQLITAASL VCRKRKGTEV QVDDIKRVYS LFLDESRSTQ YMKEYQDAFL FNELKGETMD TS
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions:
Concentration of Human RuvB-like 2 Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant Human RuvB-like 2 Protein General Information | |
|---|---|
| Alternate Names | |
| ECP51; CGI-46; TIH2; RVB2; TAP54-beta; TIP49B; TIP48; ECP-51; INO80J; REPTIN | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | RUVBL2 |
| Entrez Gene ID | 10856 |
| Ensemble Gene ID | ENSG00000183207 |
| RefSeq Protein Accession(s) | NP_001308120.1 |
| RefSeq mRNA Accession(s) | NM_001321191.1, NM_006666.2, XM_011526330.1 |
| UniProt ID(s) | Q9Y230 |
| UniGene ID(s) | Hs.515846 |
| HGNC ID(s) | HGNC:10475 |
| COSMIC ID Link(s) | RUVBL2 |
| KEGG Gene ID(s) | hsa:10856 |
| PharmGKB ID(s) | PA34888 |
| General Description of Recombinant Human RuvB-like 2 Protein. | |
| Possesses single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (5′ to 3′) activity; hexamerization is thought to be critical for ATP hydrolysis and adjacent subunits in the ring-like structure contribute to the ATPase activity. Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome – DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. Component of a SWR1-like complex that specifically mediates the removal of histone H2A.Z/H2AFZ from the nucleosome. Proposed core component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair. Plays an essential role in oncogenic transformation by MYC and also modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex. May also inhibit the transcriptional activity of ATF2. Involved in the endoplasmic reticulum (ER)-associated degradation (ERAD) pathway where it negatively regulates expression of ER stress response genes. |
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Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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