PDF DatasheetHuman beta-Catenin / CTNNB1 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant beta-Catenin / CTNNB1 based upon sequence from: Human
Host: QP8311 protein expressed in E. coli.
Tag: GST
Protein Construction: A DNA sequence encoding the Homo sapiens (Human) beta-Catenin / CTNNB1, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Application Notes: Please contact us for application specific information for QP8311.
Bioactivity Data: Untested
Full Length? Full Length
Expression Region: Ala2 – Leu781
Amino Acid Sequence: ATQADLMELD MAMEPDRKAA VSHWQQQSYL DSGIHSGATT TAPSLSGKGN PEEEDVDTSQ VLYEWEQGFS QSFTQEQVAD IDGQYAMTRA QRVRAAMFPE TLDEGMQIPS TQFDAAHPTN VQRLAEPSQM LKHAVVNLIN YQDDAELATR AIPELTKLLN DEDQVVVNKA AVMVHQLSKK EASRHAIMRS PQMVSAIVRT MQNTNDVETA RCTAGTLHNL SHHREGLLAI FKSGGIPALV KMLGSPVDSV LFYAITTLHN LLLHQEGAKM AVRLAGGLQK MVALLNKTNV KFLAITTDCL QILAYGNQES KLIILASGGP QALVNIMRTY TYEKLLWTTS RVLKVLSVCS SNKPAIVEAG GMQALGLHLT DPSQRLVQNC LWTLRNLSDA ATKQEGMEGL LGTLVQLLGS DDINVVTCAA GILSNLTCNN YKNKMMVCQV GGIEALVRTV LRAGDREDIT EPAICALRHL TSRHQEAEMA QNAVRLHYGL PVVVKLLHPP SHWPLIKATV GLIRNLALCP ANHAPLREQG AIPRLVQLLV RAHQDTQRRT SMGGTQQQFV EGVRMEEIVE GCTGALHILA RDVHNRIVIR GLNTIPLFVQ LLYSPIENIQ RVAAGVLCEL AQDKEAAEAI EAEGATAPLT ELLHSRNEGV ATYAAAVLFR MSEDKPQDYK KRLSVELTSS LFRTEPMAWN ETADLGLDIG AQGEPLGYRQ DDPSYRSFHS GGYGQDALGM DPMMEHEMGG HHPGADYPVD GLPDLGHAQD LMDGLPPGDS NQLAWFDTDL
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions:
Concentration of Human beta-Catenin / CTNNB1 Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant Human beta-Catenin / CTNNB1 Protein General Information | |
|---|---|
| Alternate Names | |
| beta-Catenin; CTNNB1; CTNNB; MRD19; armadillo | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | CTNNB1 |
| Entrez Gene ID | 1499 |
| Ensemble Gene ID | ENSG00000168036 |
| RefSeq Protein Accession(s) | NP_001091679.1 |
| RefSeq mRNA Accession(s) | NM_001098209.1, NM_001098210.1, NM_001904.3, XM_005264886.2, XM_017005738.1 |
| UniProt ID(s) | P35222 |
| UniGene ID(s) | Hs.476018 |
| HGNC ID(s) | HGNC:2514 |
| COSMIC ID Link(s) | CTNNB1 |
| KEGG Gene ID(s) | hsa:1499 |
| PharmGKB ID(s) | PA27013 |
| General Description of Recombinant Human beta-Catenin / CTNNB1 Protein. | |
| Key downstream component of the canonical Wnt signaling pathway. In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes. Involved in the regulation of cell adhesion. Acts as a negative regulator of centrosome cohesion. Involved in the CDK2/PTPN6/CTNNB1/CEACAM1 pathway of insulin internalization. Blocks anoikis of malignant kidney and intestinal epithelial cells and promotes their anchorage-independent growth by down-regulating DAPK2. Disrupts PML function and PML-NB formation by inhibiting RANBP2-mediated sumoylation of PML . Promotes neurogenesis by maintaining sympathetic neuroblasts within the cell cycle . | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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