PDF DatasheetHuman ADAR Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant ADAR based upon sequence from: Human
Host: QP8333 protein expressed in E. coli.
Tag: GST
Protein Construction: A DNA sequence encoding the Homo sapiens (Human) ADAR, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Application Notes: Please contact us for application specific information for QP8333.
Bioactivity Data: Untested
Full Length? Partial (see sequence information for more details).
Expression Region: Met1 – Leu176
Amino Acid Sequence: MNPRQGYSLS GYYTHPFQGY EHRQLRYQQP GPGSSPSSFL LKQIEFLKGQ LPEAPVIGKQ TPSLPPSLPG LRPRFPVLLA SSTRGRQVDI RGVPRGVHLR SQGLQRGFQH PSPRGRSLPQ RGVDCLSSHF QELSIYQDQE QRILKFLEEL GEGKATTAHD LSGKLGTPKK EINRVL
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions:
Concentration of Human ADAR Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant Human ADAR Protein General Information | |
|---|---|
| Alternate Names | |
| AGS6; ADAR1; DSH; P136; IFI4; DRADA; DSRAD; G1P1; K88DSRBP; IFI-4; Double-stranded RNA-specific adenosine deamina | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | ADAR |
| Entrez Gene ID | 103 |
| Ensemble Gene ID | ENSG00000160710 |
| RefSeq Protein Accession(s) | NP_001020278.1 |
| RefSeq mRNA Accession(s) | NM_001025107.2, NM_001111.4, NM_001193495.1, NM_015840.3, NM_015841.3, XM_006711111.3, XM_006711112.2, XM_006711113.2 |
| UniProt ID(s) | P55265 |
| UniGene ID(s) | Hs.12341 |
| HGNC ID(s) | HGNC:225 |
| COSMIC ID Link(s) | ADAR |
| KEGG Gene ID(s) | hsa:103 |
| PharmGKB ID(s) | PA24555 |
| General Description of Recombinant Human ADAR Protein. | |
| Catalyzes the hydrolytic deamination of adenosine to inosine in double-stranded RNA (dsRNA) referred to as A-to-I RNA editing. This may affect gene expression and function in a number of ways that include mRNA translation by changing codons and hence the amino acid sequence of proteins; pre-mRNA splicing by altering splice site recognition sequences; RNA stability by changing sequences involved in nuclease recognition; genetic stability in the case of RNA virus genomes by changing sequences during viral RNA replication; and RNA structure-dependent activities such as microRNA production or targeting or protein-RNA interactions. Can edit both viral and cellular RNAs and can edit RNAs at multiple sites (hyper-editing) or at specific sites (site-specific editing). Its cellular RNA substrates include: bladder cancer-associated protein (BLCAP), neurotransmitter receptors for glutamate (GRIA2) and serotonin (HTR2C) and GABA receptor (GABRA3). Site-specific RNA editing of transcripts encoding these proteins results in amino acid substitutions which consequently alters their functional activities. Exhibits low-level editing at the GRIA2 Q/R site, but edits efficiently at the R/G site and HOTSPOT1. Its viral RNA substrates include: hepatitis C virus (HCV), vesicular stomatitis virus (VSV), measles virus (MV), hepatitis delta virus (HDV), and human immunodeficiency virus type 1 (HIV-1). Exhibits either a proviral (HDV, MV, VSV and HIV-1) or an antiviral effect (HCV) and this can be editing-dependent (HDV and HCV), editing-independent (VSV and MV) or both (HIV-1). Impairs HCV replication via RNA editing at multiple sites. Enhances the replication of MV, VSV and HIV-1 through an editing-independent mechanism via suppression of EIF2AK2/PKR activation and function. Stimulates both the release and infectivity of HIV-1 viral particles by an editing-dependent mechanism where it associates with viral RNAs and edits adenosines in the 5’UTR and the Rev and Tat coding sequence. Can enhance viral replication of HDV via A-to-I editing at a site designated as amber/W, thereby changing an UAG amber stop codon to an UIG tryptophan (W) codon that permits synthesis of the large delta antigen (L-HDAg) which has a key role in the assbly of viral particles. However, high levels of ADAR1 inhibit HDV replication | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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