Human Replication protein A 32 kDa subunit Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant Replication protein A 32 kDa subunit based upon sequence from: Human
Host: QP8374 protein expressed in E. coli.
Tag: GST
Protein Construction: A DNA sequence encoding the Homo sapiens (Human) Replication protein A 32 kDa subunit, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Application Notes: Please contact us for application specific information for QP8374.
Bioactivity Data: Untested
Full Length? Partial (see sequence information for more details).
Expression Region: Met1 – Thr267
Amino Acid Sequence: MWNSGFESYG SSSYGGAGGY TQSPGGFGSP APSQAEKKSR ARAQHIVPCT ISQLLSATLV DEVFRIGNVE ISQVTIVGII RHAEKAPTNI VYKIDDMTAA PMDVRQWVDT DDTSSENTVV PPETYVKVAG HLRSFQNKKS LVAFKIMPLE DMNEFTTHIL EVINAHMVLS KANSQPSAGR APISNPGMSE AGNFGGNSFM PANGLTVAQN QVLNLIKACP RPEGLNFQDL KNQLKHMSVS SIKQAVDFLS NEGHIYSTVD DDHFKST
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions:
Concentration of Human Replication protein A 32 kDa subunit Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
Recombinant Human Replication protein A 32 kDa subunit Protein General Information | |
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Alternate Names | |
RP-A p32; RPA32; RP-A p34; REPA2 | |
Curated Database and Bioinformatic Data | |
Gene Symbol | RPA2 |
Entrez Gene ID | 6118 |
Ensemble Gene ID | ENSG00000117748 |
RefSeq Protein Accession(s) | NP_001273005.1 |
RefSeq mRNA Accession(s) | NM_001286076.1, NM_001297558.1, NM_002946.4 |
UniProt ID(s) | P15927 |
UniGene ID(s) | Hs.79411 |
HGNC ID(s) | HGNC:10290 |
COSMIC ID Link(s) | RPA2 |
KEGG Gene ID(s) | hsa:6118 |
PharmGKB ID(s) | PA34652 |
General Description of Recombinant Human Replication protein A 32 kDa subunit Protein. | |
As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Plays also a role in base excision repair (BER) probably through interaction with UNG. Through RFWD3 may activate CHEK1 and play a role in replication checkpoint control. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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