PDF DatasheetHuman X-ray repair cross-complementing protein 6 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant X-ray repair cross-complementing protein 6 based upon sequence from: Human
Host: QP8378 protein expressed in E. coli.
Tag: His
Protein Construction: A DNA sequence encoding the Homo sapiens (Human) X-ray repair cross-complementing protein 6, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Application Notes: Please contact us for application specific information for QP8378.
Bioactivity Data: Untested
Full Length? Partial (see sequence information for more details).
Expression Region: Ser6 – Ser222
Amino Acid Sequence: SYYKTEGDEE AEEEQEENLE ASGDYKYSGR DSLIFLVDAS KAMFESQSED ELTPFDMSIQ CIQSVYISKI ISSDRDLLAV VFYGTEKDKN SVNFKNIYVL QELDNPGAKR ILELDQFKGQ QGQKRFQDMM GHGSDYSLSE VLWVCANLFS DVQFKMSHKR IMLFTNEDNP HGNDSAKASR ARTKAGDLRD TGIFLDLMHL KKPGGFDISL FYRDIIS
Purity: Greater than 80% as determined by SDS-PAGE.
Reconstitution Instructions:
Concentration of Human X-ray repair cross-complementing protein 6 Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant Human X-ray repair cross-complementing protein 6 Protein General Information | |
|---|---|
| Alternate Names | |
| CTC75; ML8; KU70; TLAA; CTCBF; G22P1 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | XRCC6 |
| Entrez Gene ID | 2547 |
| Ensemble Gene ID | ENSG00000196419 |
| RefSeq Protein Accession(s) | NP_001275905.1 |
| RefSeq mRNA Accession(s) | NM_001288976.1, NM_001288977.1, NM_001469.4 |
| UniProt ID(s) | P12956 |
| UniGene ID(s) | Hs.292493 |
| HGNC ID(s) | HGNC:4055 |
| COSMIC ID Link(s) | XRCC6 |
| KEGG Gene ID(s) | hsa:2547 |
| PharmGKB ID(s) | PA28467 |
| General Description of Recombinant Human X-ray repair cross-complementing protein 6 Protein. | |
| Single-stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3′-5′ direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing th together. The assbly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. Required for osteocalcin gene expression. Probably also acts as a 5′-deoxyribose-5-phosphate lyase (5′-dRP lyase), by catalyzing the beta-elimination of the 5′ deoxyribose-5-phosphate at an abasic site near double-strand breaks. 5′-dRP lyase activity allows to ‘clean’ the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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