PDF DatasheetHuman OTUB1 / OTB1 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant OTUB1 / OTB1 based upon sequence from: Human
Host: QP8451 protein expressed in E. coli.
Tag: GST
Protein Construction: A DNA sequence encoding the Homo sapiens (Human) OTUB1 / OTB1, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Application Notes: Please contact us for application specific information for QP8451.
Bioactivity Data: Untested
Full Length? Partial (see sequence information for more details).
Expression Region: Ala2 – Lys201
Amino Acid Sequence: AAEEPQQQKQ EPLGSDSEGV NCLAYDEAIM AQQDRIQQEI AVQNPLVSER LELSVLYKEY AEDDNIYQQK IKDLHKKYSY IRKTRPDGNC FYRAFGFSHL EALLDDSKEL QRFKAVSAKS KEDLVSQGFT EFTIEDFHNT FMDLIEQVEK QTSVADLLAS FNDQSTSDYL VVYLRLLTSG YLQRESKFFE HFIEGGRTVK
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions:
Concentration of Human OTUB1 / OTB1 Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant Human OTUB1 / OTB1 Protein General Information | |
|---|---|
| Alternate Names | |
| OTUB1; OTB1; OTB1; HSPC263; OTU1 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | OTUB1 |
| Entrez Gene ID | 55611 |
| Ensemble Gene ID | ENSG00000167770 |
| RefSeq Protein Accession(s) | NP_060140.2 |
| RefSeq mRNA Accession(s) | NM_017670.2 |
| UniProt ID(s) | Q96FW1 |
| UniGene ID(s) | Hs.473788 |
| HGNC ID(s) | HGNC:23077 |
| COSMIC ID Link(s) | OTUB1 |
| KEGG Gene ID(s) | hsa:55611 |
| PharmGKB ID(s) | PA134988141 |
| General Description of Recombinant Human OTUB1 / OTB1 Protein. | |
| Hydrolase that can specifically rove ‘Lys-48’-linked conjugated ubiquitin from proteins and plays an important regulatory role at the level of protein turnover by preventing degradation. Regulator of T-cell anergy, a phenomenon that occurs when T-cells are rendered unresponsive to antigen rechallenge and no longer respond to their cognate antigen. Acts via its interaction with RNF128/GRAIL, a crucial inductor of CD4 T-cell anergy. Isoform 1 destabilizes RNF128, leading to prevent anergy. In contrast, isoform 2 stabilizes RNF128 and promotes anergy. Surprisingly, it regulates RNF128-mediated ubiquitination, but does not deubiquitinate polyubiquitinated RNF128. Deubiquitinates estrogen receptor alpha (ESR1). Mediates deubiquitination of ‘Lys-48’-linked polyubiquitin chains, but not ‘Lys-63’-linked polyubiquitin chains. Not able to cleave di-ubiquitin. Also capable of roving NEDD8 from NEDD8 conjugates, but with a much lower preference compared to ‘Lys-48’-linked ubiquitin.Plays a key non-catalytic role in DNA repair regulation by inhibiting activity of RNF168, an E3 ubiquitin-protein ligase that promotes accumulation of ‘Lys-63’-linked histone H2A and H2AX at DNA damage sites. Inhibits RNF168 independently of ubiquitin thioesterase activity by binding and inhibiting UBE2N/UBC13, the E2 partner of RNF168, thereby limiting spreading of ‘Lys-63’-linked histone H2A and H2AX marks. Inhibition occurs by binding to free ubiquitin: free ubiquitin acts as an allosteric regulator that increases affinity for UBE2N/UBC13 and disrupts interaction with UBE2V1. The OTUB1-UBE2N/UBC13-free ubiquitin complex adopts a configuration that mimics a cleaved ‘Lys48’-linked di-ubiquitin chain. | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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