PDF DatasheetHuman Transcription intermediary factor 1-beta Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant Transcription intermediary factor 1-beta based upon sequence from: Human
Host: QP8501 protein expressed in E. coli.
Tag: GST
Protein Construction: A DNA sequence encoding the Homo sapiens (Human) Transcription intermediary factor 1-beta, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Application Notes: Please contact us for application specific information for QP8501.
Bioactivity Data: Untested
Full Length? Partial (see sequence information for more details).
Expression Region: Pro22 – Val291
Amino Acid Sequence: PGEGSAGGEK RSTAPSAAAS ASASAAASSP AGGGAEALEL LEHCGVCRER LRPEREPRLL PCLHSACSAC LGPAAPAAAN SSGDGGAAGD GTVVDCPVCK QQCFSKDIVE NYFMRDSGSK AATDAQDANQ CCTSCEDNAP ATSYCVECSE PLCETCVEAH QRVKYTKDHT VRSTGPAKSR DGERTVYCNV HKHEPLVLFC ESCDTLTCRD CQLNAHKDHQ YQFLEDAVRN QRKLLASLVK RLGDKHATLQ KSTKEVRSSI RQVSDVQKRV
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions:
Concentration of Human Transcription intermediary factor 1-beta Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant Human Transcription intermediary factor 1-beta Protein General Information | |
|---|---|
| Alternate Names | |
| KAP1; PPP1R157; RNF96; TF1B; TIF1B | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | TRIM28 |
| Entrez Gene ID | 10155 |
| Ensemble Gene ID | ENSG00000130726 |
| RefSeq Protein Accession(s) | NP_005753.1 |
| RefSeq mRNA Accession(s) | NM_005762.2 |
| UniProt ID(s) | Q13263 |
| UniGene ID(s) | Hs.467408 |
| HGNC ID(s) | HGNC:16384 |
| COSMIC ID Link(s) | TRIM28 |
| KEGG Gene ID(s) | hsa:10155 |
| PharmGKB ID(s) | PA38131 |
| General Description of Recombinant Human Transcription intermediary factor 1-beta Protein. | |
| Nuclear corepressor for KRAB domain-containing zinc finger proteins (KRAB-ZFPs). Mediates gene silencing by recruiting CHD3, a subunit of the nucleosome rodeling and deacetylation (NuRD) complex, and SETDB1 (which specifically methylates histone H3 at ‘Lys-9’ (H3K9me)) to the promoter regions of KRAB target genes. Enhances transcriptional repression by coordinating the increase in H3K9me, the decrease in histone H3 ‘Lys-9 and ‘Lys-14’ acetylation (H3K9ac and H3K14ac, respectively) and the disposition of HP1 proteins to silence gene expression. Recruitment of SETDB1 induces heterochromatinization. May play a role as a coactivator for CEBPB and NR3C1 in the transcriptional activation of ORM1. Also corepressor for ERBB4. Inhibits E2F1 activity by stimulating E2F1-HDAC1 complex formation and inhibiting E2F1 acetylation. May serve as a partial backup to prevent E2F1-mediated apoptosis in the absence of RB1. Important regulator of CDKN1A/p21(CIP1). Has E3 SUMO-protein ligase activity toward itself via its PHD-type zinc finger. Also specifically sumoylates IRF7, thereby inhibiting its transactivation activity. Ubiquitinates p53/TP53 leading to its proteosomal degradation; the function is enhanced by MAGEC2 and MAGEA2, and possibly MAGEA3 and MAGEA6. Mediates the nuclear localization of KOX1, ZNF268 and ZNF300 transcription factors. In association with isoform 2 of ZFP90, is required for the transcriptional repressor activity of FOXP3 and the suppressive function of regulatory T-cells (Treg) | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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