PDF DatasheetE. coli 30S ribosomal protein S4 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant 30S ribosomal protein S4 based upon sequence from: E. coli
Host: QP8613 protein expressed in E. coli.
Tag: His
Protein Construction: A DNA sequence encoding the Escherichia coli (strain K12) 30S ribosomal protein S4, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Application Notes: Please contact us for application specific information for QP8613.
Bioactivity Data: Untested
Full Length? Full Length
Expression Region: Ala2 – Lys206
Amino Acid Sequence: ARYLGPKLKL SRREGTDLFL KSGVRAIDTK CKIEQAPGQH GARKPRLSDY GVQLREKQKV RRIYGVLERQ FRNYYKEAAR LKGNTGENLL ALLEGRLDNV VYRMGFGATR AEARQLVSHK AIMVNGRVVN IASYQVSPND VVSIREKAKK QSRVKAALEL AEQREKPTWL EVDAGKMEGT FKRKPERSDL SADINEHLIV ELYSK
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions:
Concentration of E. coli 30S ribosomal protein S4 Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant E. coli 30S ribosomal protein S4 Protein General Information | |
|---|---|
| Alternate Names | |
| ECK3283; JW3258; sud; ramA; b3296 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | rpsD |
| Entrez Gene ID | 32366218 |
| RefSeq Protein Accession(s) | NP_417755.1 |
| RefSeq mRNA Accession(s) | NC_000913.3, NZ_LN832404.1 |
| UniProt ID(s) | P0A7V8 |
| KEGG Gene ID(s) | ecj:JW3258 |
| General Description of Recombinant E. coli 30S ribosomal protein S4 Protein. | |
| One of two assbly initiator proteins for the 30S subunit, it binds directly to 16S rRNA where it nucleates assbly of the body of the 30S subunit.With S5 and S12 plays an important role in translational accuracy; many suppressors of streptomycin-dependent mutants of protein S12 are found in this protein, some but not all of which decrease translational accuracy (ram, ribosomal ambiguity mutations).Plays a role in mRNA unwinding by the ribosome, possibly by forming part of a processivity clamp.Protein S4 is also a translational repressor protein, it controls the translation of the alpha-operon (which codes for S13, S11, S4, RNA polymerase alpha subunit, and L17) by binding to its mRNA.Also functions as a rho-dependent antiterminator of rRNA transcription, increasing the synthesis of rRNA under conditions of excess protein, allowing a more rapid return to homeostasis. Binds directly to RNA polymerase. | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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