PDF DatasheetE. coli 30S ribosomal protein S19 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant 30S ribosomal protein S19 based upon sequence from: E. coli
Host: QP8617 protein expressed in E. coli.
Tag: His
Protein Construction: A DNA sequence encoding the Escherichia coli (strain K12) 30S ribosomal protein S19, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Application Notes: Please contact us for application specific information for QP8617.
Bioactivity Data: Untested
Full Length? Full Length
Expression Region: Pro2 – Lys92
Amino Acid Sequence: PRSLKKGPFI DLHLLKKVEK AVESGDKKPL RTWSRRSTIF PNMIGLTIAV HNGRQHVPVF VTDEMVGHKL GEFAPTRTYR GHAADKKAKK K
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions:
Concentration of E. coli 30S ribosomal protein S19 Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant E. coli 30S ribosomal protein S19 Protein General Information | |
|---|---|
| Alternate Names | |
| b3316; ECK3303; JW3278 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | rpsS |
| Entrez Gene ID | 31655999 |
| RefSeq Protein Accession(s) | NP_417775.1 |
| RefSeq mRNA Accession(s) | NC_000913.3, NZ_LN832404.1 |
| UniProt ID(s) | P0A7U3 |
| KEGG Gene ID(s) | ecj:JW3278 |
| General Description of Recombinant E. coli 30S ribosomal protein S19 Protein. | |
| In the E.coli 70S ribosome in the initiation state it has been modeled to contact the 23S rRNA of the 50S subunit forming part of bridge B1a; this bridge is broken in the model with bound EF-G. The 23S rRNA contact site in bridge B1a is modeled to differ in different ribosomal states , contacting alternately S13 or S19. In the 3.5 angstroms resolved ribosome structures the contacts between L5, S13 and S19 bridge B1b are different, confirming the dynamic nature of this interaction. Bridge B1a is not visible in the crystallized ribosomes due to 23S rRNA disorder. | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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