PDF DatasheetE. coli 30S ribosomal protein S15 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant 30S ribosomal protein S15 based upon sequence from: E. coli
Host: QP8619 protein expressed in E. coli.
Tag: His
Protein Construction: A DNA sequence encoding the Escherichia coli (strain K12) 30S ribosomal protein S15, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Application Notes: Please contact us for application specific information for QP8619.
Bioactivity Data: Untested
Full Length? Full Length
Expression Region: Ser2 – Arg89
Amino Acid Sequence: SLSTEATAKI VSEFGRDAND TGSTEVQVAL LTAQINHLQG HFAEHKKDHH SRRGLLRMVS QRRKLLDYLK RKDVARYTQL IERLGLRR
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions:
Concentration of E. coli 30S ribosomal protein S15 Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant E. coli 30S ribosomal protein S15 Protein General Information | |
|---|---|
| Alternate Names | |
| b3165; JW3134; ECK3154; secC | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | rpsO |
| Entrez Gene ID | 947686 |
| RefSeq Protein Accession(s) | NP_417634.1 |
| RefSeq mRNA Accession(s) | NC_000913.3, NZ_LN832404.1 |
| UniProt ID(s) | P0ADZ4 |
| KEGG Gene ID(s) | ecj:JW3134 |
| General Description of Recombinant E. coli 30S ribosomal protein S15 Protein. | |
| One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it helps nucleate assbly of the platform of the 30S subunit by binding and bridging several RNA helices of the 16S rRNA.In the E.coli 70S ribosome it has been modeled to contact the 23S rRNA of the 50S subunit forming part of bridge B4. In the two 3.5 A resolved ribosome structures there are minor differences between side-chain conformations. | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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