PDF DatasheetE. coli ATP-dependent DNA helicase Rep Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant ATP-dependent DNA helicase Rep based upon sequence from: E. coli
Host: QP8666 protein expressed in E. coli.
Tag: GST
Protein Construction: A DNA sequence encoding the Escherichia coli (strain K12) ATP-dependent DNA helicase Rep, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Application Notes: Please contact us for application specific information for QP8666.
Bioactivity Data: Untested
Full Length? Full Length
Expression Region: Met1 – Lys673
Amino Acid Sequence: MRLNPGQQQA VEFVTGPCLV LAGAGSGKTR VITNKIAHLI RGCGYQARHI AAVTFTNKAA REMKERVGQT LGRKEARGLM ISTFHTLGLD IIKREYAALG MKANFSLFDD TDQLALLKEL TEGLIEDDKV LLQQLISTIS NWKNDLKTPS QAAASAIGER DRIFAHCYGL YDAHLKACNV LDFDDLILLP TLLLQRNEEV RKRWQNKIRY LLVDEYQDTN TSQYELVKLL VGSRARFTVV GDDDQSIYSW RGARPQNLVL LSQDFPALKV IKLEQNYRSS GRILKAANIL IANNPHVFEK RLFSELGYGA ELKVLSANNE EHEAERVTGE LIAHHFVNKT QYKDYAILYR GNHQSRVFEK FLMQNRIPYK ISGGTSFFSR PEIKDLLAYL RVLTNPDDDS AFLRIVNTPK REIGPATLKK LGEWAMTRNK SMFTASFDMG LSQTLSGRGY EALTRFTHWL AEIQRLAERE PIAAVRDLIH GMDYESWLYE TSPSPKAAEM RMKNVNQLFS WMTEMLEGSE LDEPMTLTQV VTRFTLRDMM ERGESEEELD QVQLMTLHAS KGLEFPYVYM VGMEEGFLPH QSSIDEDNID EERRLAYVGI TRAQKELTFT LCKERRQYGE LVRPEPSRFL LELPQDDLIW EQERKVVSAE ERMQKGQSHL ANLKAMMAAK RGK
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions:
Concentration of E. coli ATP-dependent DNA helicase Rep Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant E. coli ATP-dependent DNA helicase Rep Protein General Information | |
|---|---|
| Alternate Names | |
| JW5604; dasC; mmrA?; ECK3770; mbrA; b3778 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | rep |
| Entrez Gene ID | 948292 |
| RefSeq Protein Accession(s) | WP_001238899.1 |
| RefSeq mRNA Accession(s) | NZ_LN832404.1, NC_000913.3 |
| UniProt ID(s) | P09980 |
| KEGG Gene ID(s) | ecj:JW5604 |
| General Description of Recombinant E. coli ATP-dependent DNA helicase Rep Protein. | |
| Rep helicase is a single-stranded DNA-dependent ATPase involved in DNA replication; it can initiate unwinding at a nick in the DNA. It binds to the single-stranded DNA and acts in a progressive fashion along the DNA in the 3′ to 5′ direction. | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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