PDF DatasheetHuman Telomerase reverse transcriptase Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant Telomerase reverse transcriptase based upon sequence from: Human
Host: QP8700 protein expressed in E. coli.
Tag: His
Protein Construction: A DNA sequence encoding the Homo sapiens (Human) Telomerase reverse transcriptase, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Application Notes: Please contact us for application specific information for QP8700.
Bioactivity Data: Untested
Full Length? Partial (see sequence information for more details).
Expression Region: Glu281 – Ala436
Amino Acid Sequence: EATSLEGALS GTRHSHPSVG RQHHAGPPST SRPPRPWDTP CPPVYAETKH FLYSSGDKEQ LRPSFLLSSL RPSLTGARRL VETIFLGSRP WMPGTPRRLP RLPQRYWQMR PLFLELLGNH AQCPYGVLLK THCPLRAAVT PAAGVCAREK PQGSVA
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions:
Concentration of Human Telomerase reverse transcriptase Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant Human Telomerase reverse transcriptase Protein General Information | |
|---|---|
| Alternate Names | |
| EST2; TP2; DKCA2; hTRT; CMM9; TCS1; hEST2; DKCB4; PFBMFT1; TRT | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | TERT |
| Entrez Gene ID | 7015 |
| Ensemble Gene ID | ENSG00000164362 |
| RefSeq Protein Accession(s) | NP_001180305.1 |
| RefSeq mRNA Accession(s) | NM_001193376.1, NM_198253.2 |
| UniProt ID(s) | O14746 |
| UniGene ID(s) | Hs.492203 |
| HGNC ID(s) | HGNC:11730 |
| COSMIC ID Link(s) | TERT |
| KEGG Gene ID(s) | hsa:7015 |
| PharmGKB ID(s) | PA36447 |
| General Description of Recombinant Human Telomerase reverse transcriptase Protein. | |
| Telomerase is a ribonucleoprotein enzyme essential for the replication of chromosome termini in most eukaryotes. Active in progenitor and cancer cells. Inactive, or very low activity, in normal somatic cells. Catalytic component of the teleromerase holoenzyme complex whose main activity is the elongation of telomeres by acting as a reverse transcriptase that adds simple sequence repeats to chromosome ends by copying a tplate sequence within the RNA component of the enzyme. Catalyzes the RNA-dependent extension of 3′-chromosomal termini with the 6-nucleotide telomeric repeat unit, 5′-TTAGGG-3′. The catalytic cycle involves primer binding, primer extension and release of product once the tplate boundary has been reached or nascent product translocation followed by further extension. More active on substrates containing 2 or 3 telomeric repeats. Telomerase activity is regulated by a number of factors including telomerase complex-associated proteins, chaperones and polypeptide modifiers. Modulates Wnt signaling. Plays important roles in aging and antiapoptosis. | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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