PDF DatasheetHuman Estrogen Receptor 1 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant Estrogen Receptor 1 based upon sequence from: Human
Host: QP8714 protein expressed in E. coli.
Tag: His
Protein Construction: A DNA sequence encoding the Homo sapiens (Human) Estrogen Receptor 1, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Application Notes: Please contact us for application specific information for QP8714.
Bioactivity Data: Untested
Full Length? Partial (see sequence information for more details).
Expression Region: Ala9 – Phe591
Amino Acid Sequence: ASGMALLHQI QGNELEPLNR PQLKIPLERP LGEVYLDSSK PAVYNYPEGA AYEFNAAAAA NAQVYGQTGL PYGPGSEAAA FGSNGLGGFP PLNSVSPSPL MLLHPPPQLS PFLQPHGQQV PYYLENEPSG YTVREAGPPA FYRPNSDNRR QGGRERLAST NDKGSMAMES AKETRYCAVC NDYASGYHYG VWSCEGCKAF FKRSIQGHND YMCPATNQCT IDKNRRKSCQ ACRLRKCYEV GMMKGGIRKD RRGGRMLKHK RQRDDGEGRG EVGSAGDMRA ANLWPSPLMI KRSKKNSLAL SLTADQMVSA LLDAEPPILY SEYDPTRPFS EASMMGLLTN LADRELVHMI NWAKRVPGFV DLTLHDQVHL LECAWLEILM IGLVWRSMEH PGKLLFAPNL LLDRNQGKCV EGMVEIFDML LATSSRFRMM NLQGEEFVCL KSIILLNSGV YTFLSSTLKS LEEKDHIHRV LDKITDTLIH LMAKAGLTLQ QQHQRLAQLL LILSHIRHMS NKGMEHLYSM KCKNVVPLYD LLLEMLDAHR LHAPTSRGGA SVEETDQSHL ATAGSTSSHS LQKYYITGEA EGF
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions:
Concentration of Human Estrogen Receptor 1 Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant Human Estrogen Receptor 1 Protein General Information | |
|---|---|
| Alternate Names | |
| Estrogen Receptor 1; ESRA; ESR; NR3A1; ER; ESTRR; Era | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | ESR1 |
| Entrez Gene ID | 2099 |
| Ensemble Gene ID | ENSG00000091831 |
| RefSeq Protein Accession(s) | NP_000116.2 |
| RefSeq mRNA Accession(s) | NM_000125.3, NM_001122740.1, NM_001122741.1, NM_001122742.1, NM_001291230.1, NM_001291241.1, NM_001328100.1, XM_006715375.3, XM_011535543.2, XM_011535544.2, XM_011535545.2, XM_017010376.1, XM_017010377.1, XM_017010378.1, XM_017010379.1, XM_017010380.1, XM_017010381.1 |
| UniProt ID(s) | P03372 |
| UniGene ID(s) | Hs.208124 |
| HGNC ID(s) | HGNC:3467 |
| COSMIC ID Link(s) | ESR1 |
| KEGG Gene ID(s) | hsa:2099 |
| PharmGKB ID(s) | PA156 |
| General Description of Recombinant Human Estrogen Receptor 1 Protein. | |
| Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Ligand-dependent nuclear transactivation involves either direct homodimer binding to a palindromic estrogen response elent (ERE) sequence or association with other DNA-binding transcription factors, such as AP-1/c-Jun, c-Fos, ATF-2, Sp1 and Sp3, to mediate ERE-independent signaling. Ligand binding induces a conformational change allowing subsequent or combinatorial association with multiprotein coactivator complexes through LXXLL motifs of their respective components. Mutual transrepression occurs between the estrogen receptor (ER) and NF-kappa-B in a cell-type specific manner. Decreases NF-kappa-B DNA-binding activity and inhibits NF-kappa-B-mediated transcription from the IL6 promoter and displace RELA/p65 and associated coregulators from the promoter. Recruited to the NF-kappa-B response elent of the CCL2 and IL8 promoters and can displace CREBBP. Present with NF-kappa-B components RELA/p65 and NFKB1/p50 on ERE sequences. Can also act synergistically with NF-kappa-B to activate transcription involving respective recruitment adjacent response elents; the function involves CREBBP. Can activate the transcriptional activity of TFF1. Also mediates mbrane-initiated estrogen signaling involving various kinase cascades. Isoform 3 is involved in activation of NOS3 and endothelial nitric oxide production. Isoforms lacking one or several functional domains are thought to modulate transcriptional activity by competitive ligand or DNA binding and/or heterodimerization with the full length receptor. Essential for MTA1-mediated transcriptional regulation of BRCA1 and BCAS3. Isoform 3 can bind to ERE and inhibit isoform 1 | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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