PDF DatasheetHuman Cyclin-dependent kinase 4 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant Cyclin-dependent kinase 4 based upon sequence from: Human
Host: QP8723 protein expressed in E. coli.
Tag: His
Protein Construction: A DNA sequence encoding the Homo sapiens (Human) Cyclin-dependent kinase 4, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Application Notes: Please contact us for application specific information for QP8723.
Bioactivity Data: Untested
Full Length? Full Length
Expression Region: Ala2 – Glu303
Amino Acid Sequence: ATSRYEPVAE IGVGAYGTVY KARDPHSGHF VALKSVRVPN GGGGGGGLPI STVREVALLR RLEAFEHPNV VRLMDVCATS RTDREIKVTL VFEHVDQDLR TYLDKAPPPG LPAETIKDLM RQFLRGLDFL HANCIVHRDL KPENILVTSG GTVKLADFGL ARIYSYQMAL TPVVVTLWYR APEVLLQSTY ATPVDMWSVG CIFAEMFRRK PLFCGNSEAD QLGKIFDLIG LPPEDDWPRD VSLPRGAFPP RGPRPVQSVV PEMEESGAQL LLEMLTFNPH KRISAFRALQ HSYLHKDEGN PE
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions:
Concentration of Human Cyclin-dependent kinase 4 Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant Human Cyclin-dependent kinase 4 Protein General Information | |
|---|---|
| Alternate Names | |
| PSK-J3; CMM3 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | CDK4 |
| Entrez Gene ID | 1019 |
| Ensemble Gene ID | ENSG00000135446 |
| RefSeq Protein Accession(s) | NP_000066.1 |
| RefSeq mRNA Accession(s) | NM_000075.3 |
| UniProt ID(s) | P11802 |
| UniGene ID(s) | Hs.95577 |
| HGNC ID(s) | HGNC:1773 |
| COSMIC ID Link(s) | CDK4 |
| KEGG Gene ID(s) | hsa:1019 |
| PharmGKB ID(s) | PA102 |
| General Description of Recombinant Human Cyclin-dependent kinase 4 Protein. | |
| Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit mbers of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G1/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G1 phase. Hypophosphorylates RB1 in early G1 phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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