PDF DatasheetMouse Egl nine homolog 3 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant Egl nine homolog 3 based upon sequence from: Mouse
Host: QP8813 protein expressed in E. coli.
Tag: His
Protein Construction: A DNA sequence encoding the Mus musculus (Mouse) Egl nine homolog 3, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Application Notes: Please contact us for application specific information for QP8813.
Bioactivity Data: Untested
Full Length? Full Length
Expression Region: Pro2 – Asp239
Amino Acid Sequence: PLGHIMRLDL EKIALEYIVP CLHEVGFCYL DNFLGEVVGD CVLERVKQLH YNGALRDGQL AGPRAGVSKR HLRGDQITWI GGNEEGCEAI NFLLSLIDRL VLYCGSRLGK YYVKERSKAM VACYPGNGTG YVRHVDNPNG DGRCITCIYY LNKNWDAKLH GGVLRIFPEG KSFVADVEPI FDRLLFFWSD RRNPHEVQPS YATRYAMTVW YFDAEERAEA KKKFRNLTRK TESALAKD
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions:
Concentration of Mouse Egl nine homolog 3 Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant Mouse Egl nine homolog 3 Protein General Information | |
|---|---|
| Alternate Names | |
| AI505553; Phd3; 2610021G09Rik; AI648162; SM-20; Hif-p4h-3 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | Egln3 |
| Entrez Gene ID | 112407 |
| RefSeq Protein Accession(s) | NP_082409.2 |
| RefSeq mRNA Accession(s) | NM_028133.2 |
| UniProt ID(s) | Q91UZ4 |
| UniGene ID(s) | Mm.133037 |
| COSMIC ID Link(s) | Egln3 |
| KEGG Gene ID(s) | mmu:112407 |
| General Description of Recombinant Mouse Egl nine homolog 3 Protein. | |
| Plays a crucial role in DNA damage response (DDR) by hydroxylating TELO2, promoting its interaction with ATR which is required for activation of the ATR/CHK1/p53 pathway . Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. Also hydroxylates HIF2A. Has a preference for the CODD site for both HIF1A and HIF2A. Hydroxylation on the NODD site by EGLN3 appears to require prior hydroxylation on the CODD site. Hydroxylated HIFs are then targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Under hypoxic conditions, the hydroxylation reaction is attenuated allowing HIFs to escape degradation resulting in their translocation to the nucleus, heterodimerization with HIF1B, and increased expression of hypoxy-inducible genes. ELGN3 is the most important isozyme in limiting physiological activation of HIFs (particularly HIF2A) in hypoxia. Also hydroxylates PKM in hypoxia, limiting glycolysis. Under normoxia, hydroxylates and regulates the stability of ADRB2. Regulator of cardiomyocyte and neuronal apoptosis. In cardiomyocytes, inhibits the anti-apoptotic effect of BCL2 by disrupting the BAX-BCL2 complex. In neurons, has a NGF-induced proapoptotic effect, probably through regulating CASP3 activity. Also essential for hypoxic regulation of neutrophilic inflammation. Target proteins are preferencially recognized via a LXXLAP motif.1 Publication | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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