PDF DatasheetRat p53 / TP53 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant p53 / TP53 based upon sequence from: Rat
Host: QP8835 protein expressed in E. coli.
Tag: His
Protein Construction: A DNA sequence encoding the Rattus norvegicus (Rat) p53 / TP53, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Application Notes: Please contact us for application specific information for QP8835.
Bioactivity Data: Untested
Full Length? Full Length
Expression Region: Met1 – Asp391
Amino Acid Sequence: MEDSQSDMSI ELPLSQETFS CLWKLLPPDD ILPTTATGSP NSMEDLFLPQ DVAELLEGPE EALQVSAPAA QEPGTEAPAP VAPASATPWP LSSSVPSQKT YQGNYGFHLG FLQSGTAKSV MCTYSISLNK LFCQLAKTCP VQLWVTSTPP PGTRVRAMAI YKKSQHMTEV VRRCPHHERC SDGDGLAPPQ HLIRVEGNPY AEYLDDRQTF RHSVVVPYEP PEVGSDYTTI HYKYMCNSSC MGGMNRRPIL TIITLEDSSG NLLGRDSFEV RVCACPGRDR RTEEENFRKK EEHCPELPPG SAKRALPTST SSSPQQKKKP LDGEYFTLKI RGRERFEMFR ELNEALELKD ARAAEESGDS RAHSSYPKTK KGQSTSRHKK PMIKKVGPDS D
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions:
Concentration of Rat p53 / TP53 Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant Rat p53 / TP53 Protein General Information | |
|---|---|
| Curated Database and Bioinformatic Data | |
| Gene Symbol | TP53 |
| Entrez Gene ID | 24842 |
| Ensemble Gene ID | ENSRNOG00000010756 |
| RefSeq Protein Accession(s) | NP_112251.2 |
| RefSeq mRNA Accession(s) | NM_030989.3, XM_006246594.3, XM_006246595.3 |
| UniProt ID(s) | P10361 |
| UniGene ID(s) | Rn.54443 |
| COSMIC ID Link(s) | TP53 |
| KEGG Gene ID(s) | rno:24842 |
| General Description of Recombinant Rat p53 / TP53 Protein. | |
| Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction ses to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and se to have to effect on cell-cycle regulation. Regulates the circadian clock by repressing CLOCK-ARNTL/BMAL1-mediated transcriptional activation of PER2. | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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