PDF DatasheetRat Retinoblastoma-associated protein Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant Retinoblastoma-associated protein based upon sequence from: Rat
Host: QP8836 protein expressed in E. coli.
Tag: His
Protein Construction: A DNA sequence encoding the Rattus norvegicus (Rat) Retinoblastoma-associated protein, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Application Notes: Please contact us for application specific information for QP8836.
Bioactivity Data: Untested
Full Length? Partial (see sequence information for more details).
Expression Region: Lys721 – Glu919
Amino Acid Sequence: KDLPHAAQET FKRVLIREEE FDSIIVFYNS VFMQRLKTNI LQYASTRPPT LSPIPHIPRS PYKFSSSPLR IPGGNIYISP LKSPYKISEG LPTPTKMTPR SRILVSIGES FGTSEKFQKI NQMVCNSDRV LKRSAEGGNP PKPLKKLRFD IEGSDEADGS KHLPAESKFQ QKLAEMTSTR TRMQKQKLND SMEISNKEE
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions:
Concentration of Rat Retinoblastoma-associated protein Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant Rat Retinoblastoma-associated protein Protein General Information | |
|---|---|
| Curated Database and Bioinformatic Data | |
| Gene Symbol | Rb1 |
| Entrez Gene ID | 24708 |
| RefSeq Protein Accession(s) | NP_058741.1 |
| RefSeq mRNA Accession(s) | NM_017045.1 |
| UniProt ID(s) | P33568 |
| UniGene ID(s) | Rn.55115 |
| COSMIC ID Link(s) | Rb1 |
| KEGG Gene ID(s) | rno:24708 |
| General Description of Recombinant Rat Retinoblastoma-associated protein Protein. | |
| Key regulator of entry into cell division that acts as a tumor suppressor. Promotes G0-G1 transition when phosphorylated by CDK3/cyclin-C. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. Controls histone H4 ‘Lys-20’ trimethylation. Inhibits the intrinsic kinase activity of TAF1. Mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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