![SDS-PAGE separation of QP8913 followed by commassie total protein stain results in a primary band consistent with reported data for Pyruvate dehydrogenase [ubiquinone]. These data demonstrate Greater than 90% as determined by SDS-PAGE.](https://cdn-enquirebio.pressidium.com/wp-content/uploads/2018/01/QP8913-Escherichia-coli-strain-K12-Pyruvate-dehydrogenase-ubiquinone-1.jpg "SDS-PAGE separation of QP8913 followed by commassie total protein stain results in a primary band consistent with reported data for Pyruvate dehydrogenase [ubiquinone]. These data demonstrate Greater than 90% as determined by SDS-PAGE.")
PDF DatasheetE. coli Pyruvate dehydrogenase [ubiquinone] Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant Pyruvate dehydrogenase [ubiquinone] based upon sequence from: E. coli
Host: QP8913 protein expressed in E. coli.
Tag: His
Protein Construction: A DNA sequence encoding the Escherichia coli (strain K12) Pyruvate dehydrogenase [ubiquinone], was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Application Notes: Please contact us for application specific information for QP8913.
Bioactivity Data: Untested
Full Length? Full Length
Expression Region: Met1 – Arg572
Amino Acid Sequence: MKQTVAAYIA KTLESAGVKR IWGVTGDSLN GLSDSLNRMG TIEWMSTRHE EVAAFAAGAE AQLSGELAVC AGSCGPGNLH LINGLFDCHR NHVPVLAIAA HIPSSEIGSG YFQETHPQEL FRECSHYCEL VSSPEQIPQV LAIAMRKAVL NRGVSVVVLP GDVALKPAPE GATMHWYHAP QPVVTPEEEE LRKLAQLLRY SSNIALMCGS GCAGAHKELV EFAGKIKAPI VHALRGKEHV EYDNPYDVGM TGLIGFSSGF HTMMNADTLV LLGTQFPYRA FYPTDAKIIQ IDINPASIGA HSKVDMALVG DIKSTLRALL PLVEEKADRK FLDKALEDYR DARKGLDDLA KPSEKAIHPQ YLAQQISHFA ADDAIFTCDV GTPTVWAARY LKMNGKRRLL GSFNHGSMAN AMPQALGAQA TEPERQVVAM CGDGGFSMLM GDFLSVVQMK LPVKIVVFNN SVLGFVAMEM KAGGYLTDGT ELHDTNFARI AEACGITGIR VEKASEVDEA LQRAFSIDGP VLVDVVVAKE ELAIPPQIKL EQAKGFSLYM LRAIISGRGD EVIELAKTNW LR
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions:
Concentration of E. coli Pyruvate dehydrogenase [ubiquinone] Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant E. coli Pyruvate dehydrogenase Protein Protein (1-572aa) General Information | |
|---|---|
| Alternate Names | |
| ECK0862; b0871; JW0855 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | poxB |
| Entrez Gene ID | 946132 |
| RefSeq Protein Accession(s) | NP_415392.1 |
| RefSeq mRNA Accession(s) | NC_000913.3, NZ_LN832404.1 |
| UniProt ID(s) | P07003 |
| KEGG Gene ID(s) | ecj:JW0855 |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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