PDF DatasheetHuman Caspase-8 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant Caspase-8 based upon sequence from: Human
Host: QP8921 protein expressed in E. coli.
Tag: His
Protein Construction: A DNA sequence encoding the Homo sapiens (Human) Caspase-8, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Application Notes: Please contact us for application specific information for QP8921.
Bioactivity Data: Untested
Full Length? Partial (see sequence information for more details).
Expression Region: Ser217 – Asp479
Amino Acid Sequence: SESQTLDKVY QMKSKPRGYC LIINNHNFAK AREKVPKLHS IRDRNGTHLD AGALTTTFEE LHFEIKPHDD CTVEQIYEIL KIYQLMDHSN MDCFICCILS HGDKGIIYGT DGQEAPIYEL TSQFTGLKCP SLAGKPKVFF IQACQGDNYQ KGIPVETDSE EQPYLEMDLS SPQTRYIPDE ADFLLGMATV NNCVSYRNPA EGTWYIQSLC QSLRERCPRG DDILTILTEV NYEVSNKDDK KNMGKQMPQP TFTLRKKLVF PSD
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions:
Concentration of Human Caspase-8 Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant Human Caspase-8 Protein General Information | |
|---|---|
| Alternate Names | |
| CAP4; MCH5; FLICE; MACH; Casp-8; ALPS2B | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | CASP8 |
| UniProt ID(s) | Q14790 |
| COSMIC ID Link(s) | CASP8 |
| General Description of Recombinant Human Caspase-8 Protein. | |
| Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-|-AMC. Likely target for the cowpox virus CRMA death inhibitory protein. Isoform 5, isoform 6, isoform 7 and isoform 8 lack the catalytic site and may interfere with the pro-apoptotic activity of the complex. | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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