PDF DatasheetHuman APEX1 / AP / APEx / Ref-1 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant APEX1 / AP / APEx / Ref-1 based upon sequence from: Human
Host: QP899 protein expressed in E.coli, Yeast..
Tag: His
Protein Construction: A DNA sequence encoding the Homo sapiens (Human) APEX1 / AP / APEx / Ref-1, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Recommended Applications: Immunogen, Protein Standard, Cell culture, or Other Cell Biology Applications.
Application Notes: Please contact us for application specific information for QP899.
Bioactivity Data: Untested
Full Length? Full Length
Expression Region: Lys32 – Leu318
Amino Acid Sequence: KNDKEAAGEG PALYEDPPDQ KTSPSGKPAT LKICSWNVDG LRAWIKKKGL DWVKEEAPDI LCLQETKCSE NKLPAELQEL PGLSHQYWSA PSDKEGYSGV GLLSRQCPLK VSYGIGDEEH DQEGRVIVAE FDSFVLVTAY VPNAGRGLVR LEYRQRWDEA FRKFLKGLAS RKPLVLCGDL NVAHEEIDLR NPKGNKKNAG FTPQERQGFG ELLQAVPLAD SFRHLYPNTP YAYTFWTYMM NARSKNVGWR LDYFLLSHSL LPALCDSKIR SKALGSDHCP ITLYLAL
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions:
Concentration of Human APEX1 / AP / APEx / Ref-1 Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant Human APEX1 / AP / APEx / Ref-1 Protein General Information | |
|---|---|
| Alternate Names | |
| APEX1, AP, APEx, Ref-1, APEX, APX, APE, APE1, APEN, HAP1, REF1 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | APEX1 |
| Entrez Gene ID | 328 |
| Ensemble Gene ID | ENSG00000100823 |
| RefSeq Protein Accession(s) | NP_001231178.1 |
| RefSeq mRNA Accession(s) | NM_001244249.1, NM_001641.3, NM_080648.2, NM_080649.2 |
| UniProt ID(s) | P27695 |
| UniGene ID(s) | Hs.73722 |
| HGNC ID(s) | HGNC:587 |
| COSMIC ID Link(s) | APEX1 |
| KEGG Gene ID(s) | hsa:328 |
| PharmGKB ID(s) | PA201059 |
| General Description of Recombinant Human APEX1 / AP / APEx / Ref-1 Protein. | |
| Multifunctional protein that plays a central role in the cellular response to oxidative stress. The two major activities of APEX1 in DNA repair and redox regulation of transcriptional factors. Functions as a apurinic/apyrimidinic (AP) endodeoxyribonuclease in the DNA base excision repair (BER) pathway of DNA lesions induced by oxidative and alkylating agents. Initiates repair of AP sites in DNA by catalyzing hydrolytic incision of the phosphodiester backbone immediately adjacent to the damage, generating a single-strand break with 5′-deoxyribose phosphate and 3′-hydroxyl ends. Does also incise at AP sites in the DNA strand of DNA/RNA hybrids, single-stranded DNA regions of R-loop structures, and single-stranded RNA molecules. Has a 3′-5′ exoribonuclease activity on mismatched deoxyribonucleotides at the 3′ termini of nicked or gapped DNA molecules during short-patch BER. Possesses a DNA 3′ phosphodiesterase activity capable of roving lesions (such as phosphoglycolate) blocking the 3′ side of DNA strand breaks. May also play a role in the epigenetic regulation of gene expression by participating in DNA dethylation. Acts as a loading factor for POLB onto non-incised AP sites in DNA and stimulates the 5′-terminal deoxyribose 5′-phosphate (dRp) excision activity of POLB. Plays a role in the protection from granzymes-mediated cellular repair leading to cell death. Also involved in the DNA cleavage step of class switch recombination (CSR). On the other hand, APEX1 also exerts reversible nuclear redox activity to regulate DNA binding affinity and transcriptional activity of transcriptional factors by controlling the redox status of their DNA-binding domain, such as the FOS/JUN AP-1 complex after exposure to IR. Involved in calcium-dependent down-regulation of parathyroid hormone (PTH) expression by binding to negative calcium response elents (nCaREs). Together with HNRNPL or the dimer XRCC5/XRCC6, associates with nCaRE, acting as an activator of transcriptional repression. Stimulates the YBX1-mediated MDR1 promoter activity, when acetylated at Lys-6 and Lys-7, leading to drug resistance. Acts also as an endoribonuclease involved in the control of single-stranded RNA metabolism. Plays a role in regulating MYC mRNA turnover by preferentially cleaving in between UA and CA dinucleotides of the MYC coding region determinant (CRD). In association with NMD1, plays a role in the rRNA quality control process during cell cycle progression. Associates, together with YBX1, on the MDR1 promoter. Together with NPM1, associates with rRNA. Binds DNA and RNA | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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