PDF DatasheetHuman Bruton Tyrosine Kinase / BTK Kinase Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant Bruton Tyrosine Kinase / BTK Kinase based upon sequence from: Human
Host: QP9013 protein expressed in Yeast.
Tag: His
Protein Construction: A DNA sequence encoding the Homo sapiens (Human) Bruton Tyrosine Kinase / BTK Kinase, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Application Notes: Please contact us for application specific information for QP9013.
Bioactivity Data: Untested
Full Length? Full Length
Expression Region: Ala2 – Ser659
Amino Acid Sequence: AAVILESIFL KRSQQKKKTS PLNFKKRLFL LTVHKLSYYE YDFERGRRGS KKGSIDVEKI TCVETVVPEK NPPPERQIPR RGEESSEMEQ ISIIERFPYP FQVVYDEGPL YVFSPTEELR KRWIHQLKNV IRYNSDLVQK YHPCFWIDGQ YLCCSQTAKN AMGCQILENR NGSLKPGSSH RKTKKPLPPT PEEDQILKKP LPPEPAAAPV STSELKKVVA LYDYMPMNAN DLQLRKGDEY FILEESNLPW WRARDKNGQE GYIPSNYVTE AEDSIEMYEW YSKHMTRSQA EQLLKQEGKE GGFIVRDSSK AGKYTVSVFA KSTGDPQGVI RHYVVCSTPQ SQYYLAEKHL FSTIPELINY HQHNSAGLIS RLKYPVSQQN KNAPSTAGLG YGSWEIDPKD LTFLKELGTG QFGVVKYGKW RGQYDVAIKM IKEGSMSEDE FIEEAKVMMN LSHEKLVQLY GVCTKQRPIF IITEYMANGC LLNYLREMRH RFQTQQLLEM CKDVCEAMEY LESKQFLHRD LAARNCLVND QGVVKVSDFG LSRYVLDDEY TSSVGSKFPV RWSPPEVLMY SKFSSKSDIW AFGVLMWEIY SLGKMPYERF TNSETAEHIA QGLRLYRPHL ASEKVYTIMY SCWHEKADER PTFKILLSNI LDVMDEES
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions:
Concentration of Human Bruton Tyrosine Kinase / BTK Kinase Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant Human Bruton Tyrosine Kinase / BTK Kinase Protein General Information | |
|---|---|
| Alternate Names | |
| Bruton Tyrosine Kinase; BTK Kinase; AT; ATK; XLA; AGMX1; BPK; PSCTK1; IMD1 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | Btk |
| Entrez Gene ID | 695 |
| Ensemble Gene ID | ENSG00000010671 |
| RefSeq Protein Accession(s) | NP_000052.1 |
| RefSeq mRNA Accession(s) | NM_000061.2, NM_001287344.1, NM_001287345.1 |
| UniProt ID(s) | Q06187 |
| UniGene ID(s) | Hs.159494 |
| HGNC ID(s) | HGNC:1133 |
| COSMIC ID Link(s) | Btk |
| KEGG Gene ID(s) | hsa:695 |
| PharmGKB ID(s) | PA25454 |
| General Description of Recombinant Human Bruton Tyrosine Kinase / BTK Kinase Protein. | |
| Non-receptor tyrosine kinase indispensable for B lymphocyte development, differentiation and signaling. Binding of antigen to the B-cell antigen receptor (BCR) triggers signaling that ultimately leads to B-cell activation. After BCR engagent and activation at the plasma mbrane, phosphorylates PLCG2 at several sites, igniting the downstream signaling pathway through calcium mobilization, followed by activation of the protein kinase C (PKC) family mbers. PLCG2 phosphorylation is performed in close cooperation with the adapter protein B-cell linker protein BLNK. BTK acts as a platform to bring together a diverse array of signaling proteins and is implicated in cytokine receptor signaling pathways. Plays an important role in the function of immune cells of innate as well as adaptive immunity, as a component of the Toll-like receptors (TLR) pathway. The TLR pathway acts as a primary surveillance syst for the detection of pathogens and are crucial to the activation of host defense. Especially, is a critical molecule in regulating TLR9 activation in splenic B-cells. Within the TLR pathway, induces tyrosine phosphorylation of TIRAP which leads to TIRAP degradation. BTK plays also a critical role in transcription regulation. Induces the activity of NF-kappa-B, which is involved in regulating the expression of hundreds of genes. BTK is involved on the signaling pathway linking TLR8 and TLR9 to NF-kappa-B. Transiently phosphorylates transcription factor GTF2I on tyrosine residues in response to BCR. GTF2I then translocates to the nucleus to bind regulatory enhancer elents to modulate gene expression. ARID3A and NFAT are other transcriptional target of BTK. BTK is required for the formation of functional ARID3A DNA-binding complexes. There is however no evidence that BTK itself binds directly to DNA. BTK has a dual role in the regulation of apoptosis. | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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