PDF DatasheetHuman Transcriptional repressor CTCF Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant Transcriptional repressor CTCF based upon sequence from: Human
Host: QP9086 protein expressed in Mammal, Yeast..
Tag: His
Protein Construction: A DNA sequence encoding the Homo sapiens (Human) Transcriptional repressor CTCF, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Application Notes: Please contact us for application specific information for QP9086.
Bioactivity Data: Untested
Full Length? Full Length
Expression Region: Met1 – Arg727
Amino Acid Sequence: MEGDAVEAIV EESETFIKGK ERKTYQRRRE GGQEEDACHL PQNQTDGGEV VQDVNSSVQM VMMEQLDPTL LQMKTEVMEG TVAPEAEAAV DDTQIITLQV VNMEEQPINI GELQLVQVPV PVTVPVATTS VEELQGAYEN EVSKEGLAES EPMICHTLPL PEGFQVVKVG ANGEVETLEQ GELPPQEDPS WQKDPDYQPP AKKTKKTKKS KLRYTEEGKD VDVSVYDFEE EQQEGLLSEV NAEKVVGNMK PPKPTKIKKK GVKKTFQCEL CSYTCPRRSN LDRHMKSHTD ERPHKCHLCG RAFRTVTLLR NHLNTHTGTR PHKCPDCDMA FVTSGELVRH RRYKHTHEKP FKCSMCDYAS VEVSKLKRHI RSHTGERPFQ CSLCSYASRD TYKLKRHMRT HSGEKPYECY ICHARFTQSG TMKMHILQKH TENVAKFHCP HCDTVIARKS DLGVHLRKQH SYIEQGKKCR YCDAVFHERY ALIQHQKSHK NEKRFKCDQC DYACRQERHM IMHKRTHTGE KPYACSHCDK TFRQKQLLDM HFKRYHDPNF VPAAFVCSKC GKTFTRRNTM ARHADNCAGP DGVEGENGGE TKKSKRGRKR KMRSKKEDSS DSENAEPDLD DNEDEEEPAV EIEPEPEPQP VTPAPPPAKK RRGRPPGRTN QPKQNQPTAI IQVEDQNTGA IENIIVEVKK EPDAEPAEGE EEEAQPAATD APNGDLTPEM ILSMMDR
Purity: Greater than 80% as determined by SDS-PAGE.
Reconstitution Instructions:
Concentration of Human Transcriptional repressor CTCF Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant Human Transcriptional repressor CTCF Protein General Information | |
|---|---|
| Alternate Names | |
| MRD21 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | CTCF |
| Entrez Gene ID | 10664 |
| Ensemble Gene ID | ENSG00000102974 |
| RefSeq Protein Accession(s) | NP_001177951.1 |
| RefSeq mRNA Accession(s) | NM_001191022.1, NM_006565.3, XM_017022868.1 |
| UniProt ID(s) | P49711 |
| UniGene ID(s) | Hs.368367 |
| HGNC ID(s) | HGNC:13723 |
| COSMIC ID Link(s) | CTCF |
| KEGG Gene ID(s) | hsa:10664 |
| PharmGKB ID(s) | PA26998 |
| General Description of Recombinant Human Transcriptional repressor CTCF Protein. | |
| Chromatin binding factor that binds to DNA sequence specific sites. Involved in transcriptional regulation by binding to chromatin insulators and preventing interaction between promoter and nearby enhancers and silencers. Acts as transcriptional repressor binding to promoters of vertebrate MYC gene and BAG1 gene. Also binds to the PLK and PIM1 promoters. Acts as a transcriptional activator of APP. Regulates APOA1/C3/A4/A5 gene cluster and controls MHC class II gene expression. Plays an essential role in oocyte and preimplantation embryo development by activating or repressing transcription. Seems to act as tumor suppressor. Plays a critical role in the epigenetic regulation. Participates in the allele-specific gene expression at the imprinted IGF2/H19 gene locus. On the maternal allele, binding within the H19 imprinting control region (ICR) mediates maternally inherited higher-order chromatin conformation to restrict enhancer access to IGF2. Plays a critical role in gene silencing over considerable distances in the genome. Preferentially interacts with unmethylated DNA, preventing spreading of CpG methylation and maintaining methylation-free zones. Inversely, binding to target sites is prevented by CpG methylation. Plays a important role in chromatin remodeling. Can dimerize when it is bound to different DNA sequences, mediating long-range chromatin looping. Mediates interchromosomal association between IGF2/H19 and WSB1/NF1 and may direct distant DNA segments to a common transcription factory. Causes local loss of histone acetylation and gain of histone methylation in the beta-globin locus, without affecting transcription. When bound to chromatin, it provides an anchor point for nucleosomes positioning. Seems to be essential for homologous X-chromosome pairing. May participate with Tsix in establishing a regulatable epigenetic switch for X chromosome inactivation. May play a role in preventing the propagation of stable methylation at the escape genes from X- inactivation. Involved in sister chromatid cohesion. Associates with both centromeres and chromosomal arms during metaphase and required for cohesin localization to CTCF sites. Regulates asynchronous replication of IGF2/H19. | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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