PDF DatasheetMouse Interferon regulatory factor 3 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant Interferon regulatory factor 3 based upon sequence from: Mouse
Host: QP9220 protein expressed in Yeast.
Tag: His-Myc
Protein Construction: A DNA sequence encoding the Mus musculus (Mouse) Interferon regulatory factor 3, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Application Notes: Please contact us for application specific information for QP9220.
Bioactivity Data: Untested
Full Length? Full Length
Expression Region: Met1 – Ile419
Amino Acid Sequence: METPKPRILP WLVSQLDLGQ LEGVAWLDES RTRFRIPWKH GLRQDAQMAD FGIFQAWAEA SGAYTPGKDK PDVSTWKRNF RSALNRKEVL RLAADNSKDP YDPHKVYEFV TPGARDFVHL GASPDTNGKS SLPHSQENLP KLFDGLILGP LKDEGSSDLA IVSDPSQQLP SPNVNNFLNP APQENPLKQL LAEEQWEFEV TAFYRGRQVF QQTLFCPGGL RLVGSTADMT LPWQPVTLPD PEGFLTDKLV KEYVGQVLKG LGNGLALWQA GQCLWAQRLG HSHAFWALGE ELLPDSGRGP DGEVHKDKDG AVFDLRPFVA DLIAFMEGSG HSPRYTLWFC MGEMWPQDQP WVKRLVMVKV VPTCLKELLE MAREGGASSL KTVDLHISNS QPISLTSDQY KAYLQDLVED MDFQATGNI
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions:
Concentration of Mouse Interferon regulatory factor 3 Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant Mouse Interferon regulatory factor 3 Protein General Information | |
|---|---|
| Alternate Names | |
| C920001K05Rik; IRF-3 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | Irf3 |
| Entrez Gene ID | 54131 |
| Ensemble Gene ID | ENSMUSG00000003184 |
| RefSeq Protein Accession(s) | NP_058545.1 |
| RefSeq mRNA Accession(s) | NM_016849.4, XM_006540995.3, XM_006540996.2 |
| UniProt ID(s) | P70671 |
| UniGene ID(s) | Mm.3960 |
| COSMIC ID Link(s) | Irf3 |
| KEGG Gene ID(s) | mmu:54131 |
| General Description of Recombinant Mouse Interferon regulatory factor 3 Protein. | |
| Key transcriptional regulator of type I interferon (IFN)-dependent immune responses which plays a critical role in the innate immune response against DNA and RNA viruses. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Acts as a more potent activator of the IFN-beta (IFNB) gene than the IFN-alpha (IFNA) gene and plays a critical role in both the early and late phases of the IFNA/B gene induction. Found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, is phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes. Can activate distinct gene expression programs in macrophages and can induce significant apoptosis in primary macrophages. | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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