PDF DatasheetMouse Nuclear receptor ROR-gamma Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant Nuclear receptor ROR-gamma based upon sequence from: Mouse
Host: QP9374 protein expressed in Yeast.
Tag: His
Protein Construction: A DNA sequence encoding the Mus musculus (Mouse) Nuclear receptor ROR-gamma, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Application Notes: Please contact us for application specific information for QP9374.
Bioactivity Data: Untested
Full Length? Full Length
Expression Region: Met1 – Lys516
Amino Acid Sequence: MDRAPQRHHR TSRELLAAKK THTSQIEVIP CKICGDKSSG IHYGVITCEG CKGFFRRSQQ CNVAYSCTRQ QNCPIDRTSR NRCQHCRLQK CLALGMSRDA VKFGRMSKKQ RDSLHAEVQK QLQQQQQQEQ VAKTPPAGSR GADTLTYTLG LSDGQLPLGA SPDLPEASAC PPGLLRASGS GPPYSNTLAK TEVQGASCHL EYSPERGKAE GRDSIYSTDG QLTLGRCGLR FEETRHPELG EPEQGPDSHC IPSFCSAPEV PYASLTDIEY LVQNVCKSFR ETCQLRLEDL LRQRTNLFSR EEVTSYQRKS MWEMWERCAH HLTEAIQYVV EFAKRLSGFM ELCQNDQIIL LTAGAMEVVL VRMCRAYNAN NHTVFFEGKY GGVELFRALG CSELISSIFD FSHFLSALCF SEDEIALYTA LVLINANRPG LQEKRRVEHL QYNLELAFHH HLCKTHRQGL LAKLPPKGKL RSLCSQHVEK LQIFQHLHPI VVQAAFPPLY KELFSTDVES PEGLSK
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions:
Concentration of Mouse Nuclear receptor ROR-gamma Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant Mouse Nuclear receptor ROR-gamma Protein General Information | |
|---|---|
| Alternate Names | |
| Nr1f3; RORgamma; Thor; TOR | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | RORC |
| Entrez Gene ID | 19885 |
| Ensemble Gene ID | ENSMUSG00000028150 |
| RefSeq Protein Accession(s) | NP_001280663.1 |
| RefSeq mRNA Accession(s) | NM_001293734.1, NM_011281.3 |
| UniProt ID(s) | P51450 |
| UniGene ID(s) | Mm.4372 |
| COSMIC ID Link(s) | RORC |
| KEGG Gene ID(s) | mmu:19885 |
| General Description of Recombinant Mouse Nuclear receptor ROR-gamma Protein. | |
| Nuclear receptor that binds DNA as a monomer to ROR response elements (RORE) containing a single core motif half-site 5′-AGGTCA-3′ preceded by a short A-T-rich sequence. Key regulator of cellular differentiation, immunity, peripheral circadian rhythm as well as lipid, steroid, xenobiotics and glucose metabolism. Considered to have intrinsic transcriptional activity, have some natural ligands like oxysterols that act as agonists (25-hydroxycholesterol) or inverse agonists (7-oxygenated sterols), enhancing or repressing the transcriptional activity, respectively. Recruits distinct combinations of cofactors to target gene regulatory regions to modulate their transcriptional expression, depending on the tissue, time and promoter contexts (PubMed:17666523, PubMed:19381306, PubMed:19965867, PubMed:21853531, PubMed:22789990, PubMed:23723244). Regulates the circadian expression of clock genes such as CRY1, ARNTL/BMAL1 and NR1D1 in peripheral tissues and in a tissue-selective manner (PubMed:22753030). Competes with NR1D1 for binding to their shared DNA response element on some clock genes such as ARNTL/BMAL1, CRY1 and NR1D1 itself, resulting in NR1D1-mediated repression or RORC-mediated activation of the expression, leading to the circadian pattern of clock genes expression. Therefore influences the period length and stability of the clock (PubMed:22753030). Involved in the regulation of the rhythmic expression of genes involved in glucose and lipid metabolism, including PLIN2 and AVPR1A. Negative regulator of adipocyte differentiation through the regulation of early phase genes expression, such as MMP3. Controls adipogenesis as well as adipocyte size and modulates insulin sensitivity in obesity. In liver, has specific and redundant functions with RORA as positive or negative modulator of expression of genes encoding phase I and Phase II proteins involved in the metabolism of lipids, steroids and xenobiotics, such as SULT1E1 (PubMed:21853531). Also plays also a role in the regulation of hepatocyte glucose metabolism through the regulation of G6PC and PCK1. Regulates the rhythmic expression of PROX1 and promotes its nuclear localization. | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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