PDF DatasheetHuman E3 ubiquitin-protein ligase TRIM21 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant E3 ubiquitin-protein ligase TRIM21 based upon sequence from: Human
Host: QP9447 protein expressed in Yeast.
Tag: His
Protein Construction: A DNA sequence encoding the Homo sapiens (Human) E3 ubiquitin-protein ligase TRIM21, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Application Notes: Please contact us for application specific information for QP9447.
Bioactivity Data: Untested
Full Length? Full Length
Expression Region: Met1 – Tyr475
Amino Acid Sequence: MASAARLTMM WEEVTCPICL DPFVEPVSIE CGHSFCQECI SQVGKGGGSV CPVCRQRFLL KNLRPNRQLA NMVNNLKEIS QEAREGTQGE RCAVHGERLH LFCEKDGKAL CWVCAQSRKH RDHAMVPLEE AAQEYQEKLQ VALGELRRKQ ELAEKLEVEI AIKRADWKKT VETQKSRIHA EFVQQKNFLV EEEQRQLQEL EKDEREQLRI LGEKEAKLAQ QSQALQELIS ELDRRCHSSA LELLQEVIIV LERSESWNLK DLDITSPELR SVCHVPGLKK MLRTCAVHIT LDPDTANPWL ILSEDRRQVR LGDTQQSIPG NEERFDSYPM VLGAQHFHSG KHYWEVDVTG KEAWDLGVCR DSVRRKGHFL LSSKSGFWTI WLWNKQKYEA GTYPQTPLHL QVPPCQVGIF LDYEAGMVSF YNITDHGSLI YSFSECAFTG PLRPFFSPGF NDGGKNTAPL TLCPLNIGSQ GSTDY
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions:
Concentration of Human E3 ubiquitin-protein ligase TRIM21 Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant Human E3 ubiquitin-protein ligase TRIM21 Protein General Information | |
|---|---|
| Alternate Names | |
| RNF81; Ro/SSA; RO52; SSA; SSA1 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | TRIM21 |
| Entrez Gene ID | 6737 |
| Ensemble Gene ID | ENSG00000132109 |
| RefSeq Protein Accession(s) | NP_003132.2 |
| RefSeq mRNA Accession(s) | NM_003141.3 |
| UniProt ID(s) | P19474 |
| UniGene ID(s) | Hs.532357 |
| HGNC ID(s) | HGNC:11312 |
| COSMIC ID Link(s) | TRIM21 |
| KEGG Gene ID(s) | hsa:6737 |
| PharmGKB ID(s) | PA36136 |
| General Description of Recombinant Human E3 ubiquitin-protein ligase TRIM21 Protein. | |
| E3 ubiquitin-protein ligase whose activity is dependent on E2 enzymes, UBE2D1, UBE2D2, UBE2E1 and UBE2E2. Forms a ubiquitin ligase complex in cooperation with the E2 UBE2D2 that is used not only for the ubiquitination of USP4 and IKBKB but also for its self-ubiquitination. Component of cullin-RING-based SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes such as SCF(SKP2)-like complexes. A TRIM21-containing SCF(SKP2)-like complex is shown to mediate ubiquitination of CDKN1B (‘Thr-187’ phosphorylated-form), thereby promoting its degradation by the proteasome. Monoubiquitinates IKBKB that will negatively regulates Tax-induced NF-kappa-B signaling. Negatively regulates IFN-beta production post-pathogen recognition by polyubiquitin-mediated degradation of IRF3. Mediates the ubiquitin-mediated proteasomal degradation of IgG1 heavy chain, which is linked to the VCP-mediated ER-associated degradation (ERAD) pathway. Promotes IRF8 ubiquitination, which enhanced the ability of IRF8 to stimulate cytokine genes transcription in macrophages. Plays a role in the regulation of the cell cycle progression. Enhances the decapping activity of DCP2. Exists as a ribonucleoprotein particle present in all mammalian cells studied and composed of a single polypeptide and one of four small RNA molecules. At least two isoforms are present in nucleated and red blood cells, and tissue specific differences in RO/SSA proteins have been identified. The common feature of these proteins is their ability to bind HY RNAs.2 | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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