Bacteriophage Single-stranded DNA-binding protein gp2.5 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant Single-stranded DNA-binding protein gp2.5 based upon sequence from: Bacteriophage
Host: QP9695 protein expressed in Yeast.
Tag: His
Protein Construction: A DNA sequence encoding the Enterobacteria phage T7 (Bacteriophage T7) Single-stranded DNA-binding protein gp2.5, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Application Notes: Please contact us for application specific information for QP9695.
Bioactivity Data: Untested
Full Length? Full Length
Expression Region: Met1 – Phe232
Amino Acid Sequence: MAKKIFTSAL GTAEPYAYIA KPDYGNEERG FGNPRGVYKV DLTIPNKDPR CQRMVDEIVK CHEEAYAAAV EEYEANPPAV ARGKKPLKPY EGDMPFFDNG DGTTTFKFKC YASFQDKKTK ETKHINLVVV DSKGKKMEDV PIIGGGSKLK VKYSLVPYKW NTAVGASVKL QLESVMLVEL ATFGGGEDDW ADEVEENGYV ASGSAKASKP RDEESWDEDD EESEEADEDG DF
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions:
Concentration of Bacteriophage Single-stranded DNA-binding protein gp2.5 Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
Recombinant Bacteriophage Single-stranded DNA-binding protein gp2.5 Protein General Information | |
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Alternate Names | |
T7p17 | |
Curated Database and Bioinformatic Data | |
Gene Symbol | 2.5 |
Entrez Gene ID | 1261080 |
RefSeq Protein Accession(s) | NP_041970.1 |
RefSeq mRNA Accession(s) | NC_001604.1 |
UniProt ID(s) | P03696 |
KEGG Gene ID(s) | vg:1261080 |
General Description of Recombinant Bacteriophage Single-stranded DNA-binding protein gp2.5 Protein. | |
Single-stranded DNA-binding protein that eliminates secondary structure in long ssDNA formed on the lagging strand of the replication fork. Stimulates DNA polymerase activity and increases the efficiency of RNA primer synthesis by interacting with the DNA polymerase and the helicase/primase protein gp4. Disrupts loops, hairpins and other secondary structures present on ssDNA to reduce and eliminate pausing of viral DNA polymerase at specific sites during elongation. |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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