PDF DatasheetInfluenza Polymerase acidic protein Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant Polymerase acidic protein based upon sequence from: Influenza
Host: QP9703 protein expressed in Yeast.
Tag: His
Protein Construction: A DNA sequence encoding the Influenza A virus (strain A/USA:Huston/AA/1945 H1N1) Polymerase acidic protein, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Application Notes: Please contact us for application specific information for QP9703.
Bioactivity Data: Untested
Full Length? Full Length
Expression Region: Met1 – Arg716
Amino Acid Sequence: MEDFVRQCFN PMIVELAEKA MKEYGEDLKV ETNKFAAICT HLEVCFMYSD FHFINEQGES IIVELGDPNA LLKHRFEIIE GRDRTMAWTI VNSICNTTGA EKPKFLPDLY DYKENRFIEI GVTRREVHIY YLEKANKIKS EKTHIHIFSF TGEEMATKAD YTLDEESRAR IKTRLFTIRQ EMASRGLWDS FRQSERGEET IEERFEITGT MRKLADQSLP PNFSCFENFR AYVDGFEPNG YIEGKLSQMS KEVNARIEPF LKTTPRPLKL PDGPPCSQRS KFLLMDALKL SIEDPSHEGE GIPLYDAIKC MRTFFGWKEP NVVKPHEKGI NPNYLLSWKQ VLAELQDIEN EEKIPKTKNM KKTSQLKWAL GENMAPEKVD FDDCKDVSDL KQYDSDEPEL RSLSSWIQNE FNKACELTDS IWIELDEIGE DVAPIEHIAS MRRNYFTAEV SHCRATEYIM KGVYINTALL NASCAAMDDF QLIPMISKCR TKEGRRKTNL YGFIIKGRSH LRNDTDVVNF VSMEFSLTDP RLEPHKWEKY CVLEIGDMLL RSAIGQVSRP MFLYVRTNGT SKIKMKWGME MRRCLLQSLQ QIESMIEAES SVKEKDMTKE FFENKSETWP IGESPKGVEE GSIGKVCRTL LAKSVFNSLY ASPQLEGFSA ESRKLLLIVQ ALRDNLEPGT FDLGGLYEAV EECLINDPWV LLNASWFNSF LTHALR
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions:
Concentration of Influenza Polymerase acidic protein Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant Influenza Polymerase acidic protein Protein General Information | |
|---|---|
| Curated Database and Bioinformatic Data | |
| Gene Symbol | PA |
| UniProt ID(s) | A4U6V9 |
| General Description of Recombinant Influenza Polymerase acidic protein Protein. | |
| Plays an essential role in viral RNA transcription and replication by forming the heterotrimeric polymerase complex together with PB1 and PB2 subunits. The complex transcribes viral mRNAs by using a unique mechanism called cap-snatching. It consists in the hijacking and cleavage of host capped pre-mRNAs. These short capped RNAs are then used as primers for viral mRNAs. The PB2 subunit is responsible for the binding of the 5′ cap of cellular pre-mRNAs which are subsequently cleaved after 10-13 nucleotides by the PA subunit that carries the endonuclease activity. In addition of its function in viral transcription, PA also plays an essential role in viral RNA synthesis and promotes the formation of the trimeric polymerase complex. | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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